![Western blot - Rab9 antibody [1E12] (ab68340) image](#Wb rab9 2.jpg)
![Immunocytochemistry/ Immunofluorescence - Rab9 antibody [1E12] (ab68340) image](#IF rab9.jpg)
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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Overview
Product name
Anti-Rab9 antibody
See all Rab9 products (6) ...
Description
Mouse monoclonal to Rab9
Tested applications
ELISA, WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment, corresponding to amino acids 17-116 of Human Rab9, with GST tag.
Positive control
HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab68340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 23 kDa.
ICC/IF: Use a concentration of 10 µg/ml
Target
Function
Involved in the transport of proteins between the endosomes and the trans Golgi network.
Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane.
Target information above from: UniProt accessionP51151
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2410064E05Rik antibody
- AI195561 antibody
- DmRab9 antibody
see all
Database links
- Entrez Gene: 9367 Human
- Omim: 300284 Human
- SwissProt: P51151 Human
- Unigene: 495704 Human
- Unigene: 728400 Human
Anti-Rab9 antibody images:
Western blot - Rab9 antibody (ab68340)
Anti-Rab9 antibody (ab68340) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 23 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Note: MW of GST-tag alone is 26 kDa
Western blot - Rab9 antibody [1E12] (ab68340)
![Western blot - Rab9 antibody [1E12] (ab68340)](/ps/datasheet/Images/68/ab68340/Wb rab9 2.jpg)
Anti-Rab9 antibody (ab68340) at 5 µg/ml + HeLa cell lysate at 25 µg/ml
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 23 kDa
Observed band size : 23 kDa
Additional bands at : 33 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Rab9 antibody [1E12] (ab68340)
![Immunocytochemistry/ Immunofluorescence - Rab9 antibody [1E12] (ab68340)](/ps/datasheet/Images/68/ab68340/IF rab9.jpg)
Immunofluorescence of monoclonal antibody to Rab9 ab68340 on HeLa cells. Antibody concentration 10 ug/ml.
ELISA - Rab9 antibody (ab68340)
Detection limit for recombinant GST tagged Rab9 is approximately 0.1ng/ml as a capture antibody.
References for Anti-Rab9 antibody (ab68340)
ab68340 has not yet been referenced specifically in any publications.
Publishing research using ab68340? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"