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Product name
Anti-Rab5 antibody - Drosophila Early Endosome Marker
See all Rab5 products (10) ...
Description
Rabbit polyclonal to Rab5 - Drosophila Early Endosome Marker
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Fruit fly (Drosophila melanogaster)
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Fruit fly (Drosophila melanogaster) Rab5.
(Peptide available as ab324 89.)
Positive control
This antibody gave a positive signal in Schneider L2 (Fruit fly (Drosophila melanogaster)) Whole Cell Lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Rabs
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Immunocytochemistry/ Immunofluorescence - Rab5 antibody - Drosophila Early Endosome Marker (ab31261)
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Western blot - Rab5 antibody - Drosophila Early Endosome Marker (ab31261)
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Immunocytochemistry/ Immunofluorescence - Rab5 antibody - Drosophila Early Endosome Marker (ab31261)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. PubMed: 20404143
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
Target
Function
Required for the fusion of plasma membranes and early endosomes.
Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Cellular localization
Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes.
Target information above from: UniProt accessionP20339
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- RAB 5 antibody
- RAB 5A antibody
- RAB5A antibody
see all
Anti-Rab5 antibody - Drosophila Early Endosome Marker images:
Immunocytochemistry/ Immunofluorescence - Rab5 antibody - Drosophila Early Endosome Marker (ab31261)
ab31261 staining 3rd instar wing imaginal disc cells of fruit fly (Drosophila melanogaster). Cells were formaldehyde fixed and blocked with 5 % serum for 2 hours at 22°C prior to incubating with the primary antibody, diluted 1/500, for 16 hours at 4°C. A Cy3® conjugated donkey anti-rabbit IgG (H+L) antibody, diluted 1/500, was used as the secondary.
In the image, the nuclei of some cells are marked by GFP (green). The punctate, non-nuclear staining pattern is indicative of endosomes (similar to other endosomal markers such as Hrs).
This image is courtesy of an anonymous Abreview
Western blot - Rab5 antibody - Drosophila Early Endosome Marker (ab31261)
Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261) at 1 µg/ml +
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 26 kDa
Observed band size : 26 kDa
Additional bands at : 135 kDa,145 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
ab31261 detects a strong 26 kDa band in Drosophila lysate consistent with expected molecular weight of Rab5. In some batches of this antibody, we have observed additional bands at ~135, 145 and 70 kDa, the identity of which are unknown.
Immunocytochemistry/ Immunofluorescence - Rab5 antibody - Drosophila Early Endosome Marker (ab31261)
ab31261 staining Rab5 in Drosophila S2 cells by Immunocytochemistry/ Immunofluorescence.S2 cells were fixed in 4% paraformaldehyde for 10 minutes and then permeablized by incubating with 0.2% Triton X-100 in PBS for 5 minutes at room temperature. After blocking, cells were incubated overnight with ab31261 diluted in PBS containing 10% horse serum and 0.2% Triton X-100. A Fluorescein-conjugated anti-rabbit IgG was used as the secondary.
Image from Huang HR et al, Proc Natl Acad Sci U S A. 2010 May 4;107(18):8322-7. Epub 2010 Apr 19, Fig S2. DOI 10.1073/pnas.1004031107
References for Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261)
This product has been referenced in:
- Xia R et al. USP8 promotes smoothened signaling by preventing its ubiquitination and changing its subcellular localization. PLoS Biol 10:e1001238 (2012). ICC/IF; Fruit fly (Drosophila melanogaster).Read more (PubMed: 22253573) »
- Gault WJ et al. Drosophila CK1-?, gilgamesh, controls PCP-mediated morphogenesis through regulation of vesicle trafficking. J Cell Biol 196:605-21 (2012). ICC/IF; Fruit fly (Drosophila melanogaster).Read more (PubMed: 22391037) »
See all 6 publications for this product
Publishing research using ab31261? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"