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Immunocytochemistry - Rab5 antibody - Early Endosome Marker (ab13253)
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Immunocytochemistry - Rab5 antibody - Early Endosome Marker (ab13253)
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Product Name
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Rab5 antibody - Early Endosome Marker
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See all Rab5 antibodies (9)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Rab5 - Early Endosome Marker
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Immunogen
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Synthetic peptide: PKNEPQNPGANSARGR conjugated to KLH, corresponding to amino acids 182-197 of Human Rab5 (Rab5A)
BLAST 'PKNEPQNPGANSARGR' with  or 
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Reacts with
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Hu, Ms, Rat, Cow, Hm, Mk
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Predicted to react
(species key)
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Dog
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/2000. Detects a band of approximately 26 kDa (predicted molecular weight: 25.8 kDa).
ICC/IF: Use at an assay dependent dilution (PMID 19348413).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse or Rat Brain Tissue.
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Cellular localization
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Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome.
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Research areas
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Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Rabs
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Relevance
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Rab5 is a 24 kDa GTP-binding protein that regulates the fusion of plasma membrane-derived clathrin-coated vesicles with early endosomes and homotypic fusion among early endosomes. It is localized to the cytoplasmic side of the plasma membrane, clathrin coated vesicles and early endosomes. Rab5 is believed to regulate vesicle fusion through a cycle of GDP/GTP exchange and GTP hydrolysis. The different guanine nucleotide binding states of Rab5 is postulated to affect its ability to associate or dissociate with membranes during endocytotic membrane traffic. Its GTP-bound form, which represents the active form of Rab5, associates with membrane and regulates vesicle docking and fusion. Studies using a Rab5 mutant that hydrolysed xanthosine 5'-triphosphate (XTP) indicated that nucleotide hydrolysis occurs even in the absence of membrane fusion. GTP hydrolysis by Rab5 is postulated to determine the frequency of membrane docking and fusion events.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, 1X PBS Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rab5 antibody - Early Endosome Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Rab5 antibody - Early Endosome Marker images, references, products related to ab13253 and other tools.
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Rab5 antibody - Early Endosome Marker images:
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 Immunocytochemistry - Rab5 antibody - Early Endosome Marker (ab13253)
Confocal microscopy of fixed primary cultures of rat hippocampal neurons (embryonic day 18) showing immunocytochemical labelling of polyclonal rabbit anti-Rab5 (Abcam ab13253, 1/200; Alexa Fluor 488; 1/200; green) and monoclonal mouse anti-b-tubulin-II (Alexa Fluor 568 1/200; red). Magnification 63x ; insert 180x. Randal Moldrich, CNRS UMR7637, ESPCI, France
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Immunocytochemistry - Rab5 antibody - Early Endosome Marker (ab13253)
ab13253 Rabbit polyclonal to Rab5 was used in in fixed HEK cells that had been transfected with Rab5-GFP Fluorescence microscopy of fixed Rab5-transfected HEK cells showing (left to right) immunocytochemical labelling of rabbit polyclonal Rab5 (ab13253, 1/500; Cy3; red), and Rab5-GFP (green) with their overlay (far right). Scale bar = 7µm. Showing good co-localisation. Randal Moldrich, CNRS UMR7637, ESPCI, France
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References for Rab5 antibody - Early Endosome Marker (ab13253)
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This product has been used in: (two most recent references)
McCaffrey G et al. High-resolution fractionation of signaling endosomes containing different receptors. Traffic 10:938-50 (2009). WB; Human. PubMed: 19416476
Sa(a(lik P et al. Protein Delivery with Transportans Is Mediated by Caveolae Rather Than Flotillin-Dependent Pathways. Bioconjug Chem : (2009). ICC/IF; Human. PubMed: 19348413
See all 5 publication references for this product.
If you publish research using ab13253 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Rab5
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Rab5 antibody - Early Endosome Marker - more information
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Contact Abcam with a Technical Enquiry about Rab5 antibody - Early Endosome Marker |
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