(R)-(+)-Methanandamide (ethanol solution) (ab120361)

Overview

  • Product name(R)-(+)-Methanandamide (ethanol solution)
  • Description
    Stable anandamide analogue. CB1 receptor agonist.
  • Alternative names
    • AM-356
  • Biological descriptionStable anandamide analogue. CB1 receptor agonist (Ki values are 20 and 815 nM for CB1 and CB2, respectively). Also TRPV1 agonist.
  • Purity> 98%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120361 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    (R)-(+)-Methanandamide (ethanol solution) images

    • ab134901 staining cAMP in SK-N-SH cells treated with (R)-(+)-Methanandamide (ethanol solution) (ab120361), by ICC/IF. Increase in cAMP expression correlates with increased concentration of (R)-(+)-Methanandamide (ethanol solution), as described in literature.
      The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120361 ((R)-(+)-Methanandamide (ethanol solution)) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab134901 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

    References for (R)-(+)-Methanandamide (ethanol solution) (ab120361)

    This product has been referenced in:
    • Fischer MJ & Messlinger K Cannabinoid and vanilloid effects of R(+)-methanandamide in the hemisected meningeal preparation. Cephalalgia 27:422-8 (2007). Read more (PubMed: 17448180) »
    • Järbe TU  et al. Antagonism of discriminative stimulus effects of delta(9)-THC and (R)-methanandamide in rats. Psychopharmacology (Berl) 184:36-45 (2006). Read more (PubMed: 16307294) »
    • Abadji V  et al. (R)-methanandamide: a chiral novel anandamide possessing higher potency and metabolic stability. J Med Chem 37:1889-93 (1994). Read more (PubMed: 8021930) »

    See 0 Publications for this product

    Product Wall

    ab120361 can be diluted in DMSO. Please remember to take both the solvents into consideration when it comes to controls.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"