R Phycoerythrin activated with SMCC (ab123475)

Overview

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Additional sequence information
      purified from natural source, either algae or cyanobacteria

Specifications

Our Abpromise guarantee covers the use of ab123475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Flow Cytometry

    Immunocytochemistry/ Immunofluorescence

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Liquid
  • Additional notes

    bs/Em = 565/575 nm

    Activated R-Phycoerythrin is chemically modified with SMCC. SMCC reacts with the primary amine on R-PE and introduces maleimide groups to R-PE. These maleimide groups easily react with thiol groups of target protein without the need for any additional activation, resulting in convenient conjugation of R-PE with proteins. R-PE’s primary absorption peak is at 565 nm with secondary peaks at 496 and 545 nm. The broad excitation spectrum provides the advantage for multi-color immunofluorescent staining or cell sorting.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark.

    pH: 6.00
    Constituents: 99% MES, EDTA
    Note: Contains preservative.

General Info

  • Alternative names
    • R phycoerythrin alpha subunit
    • R phycoerythrin beta subunit
    • rpeA
    • rpeB
  • Relevance
    Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
  • Cellular localization
    Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.

References

ab123475 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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