This antibody was purified from monospecific antiserum by immunoaffinity chromatography using a R Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Conjugation to magnetic particles (beads): Use at an assay dependent dilution.
ELISA: 1/20,000 - 1/100,000.
ID: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/2,000 - 1/10,000. Predicted molecular weight: 240 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances.
R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.