Anti-R Phycoerythrin antibody (Alkaline Phosphatase) (ab34729)

Overview

  • Product name
    Anti-R Phycoerythrin antibody (Alkaline Phosphatase)
    See all R Phycoerythrin primary antibodies
  • Description
    Goat polyclonal to R Phycoerythrin (Alkaline Phosphatase)
  • Conjugation
    Alkaline Phosphatase
  • Tested applications
    Suitable for: Dot blot, ELISA, WBmore details
  • Immunogen

    R-Phycoerythrin from the seaweed gracilaria.

  • General notes

    Alkaline Phosphatase (from Calf Intestine, Molecular Weight 140 kDa). Conjugation Reference: Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, 10mg/ml BSA, 0.15M Sodium chloride, 0.05M Tris HCl, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified by immunoaffinity chromatography using a R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
ELISA
WB
  • Application notes
    ELISA: 1/1,000 - 1/5,000. Assayed against 1.0 ug of R-Phycoerythrin in a standard
    capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 240 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
    • Cellular localization
      Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.
    • Alternative names
      • R phycoerythrin alpha subunit antibody
      • R phycoerythrin beta subunit antibody
      • rpeA antibody
      • rpeB antibody

    References for Anti-R Phycoerythrin antibody (Alkaline Phosphatase) (ab34729)

    ab34729 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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