Anti-R Phycoerythrin antibody (Biotin) (ab34730)

Overview

  • Product name
    Anti-R Phycoerythrin antibody (Biotin)
    See all R Phycoerythrin primary antibodies
  • Description
    Goat polyclonal to R Phycoerythrin (Biotin)
  • Conjugation
    Biotin
  • Specificity
    This antibody will cross react with B Phycoerythrin. Reactivity with other phycobiliproteins is unknown.
  • Tested applications
    Suitable for: Dot blot, WB, ELISAmore details
  • Immunogen

    Full length protein Highly purified R Phycoerythrin from the seaweed gracilaria.

  • General notes

    10-20 Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) molecules per goat IgG molecule.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
    IgG and Protease free.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using R Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent dilution.
WB 1/1000 - 1/3000. Detects a band of approximately 240 kDa.
ELISA 1/8000 - 1/32000.

Target

  • Relevance
    Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
  • Cellular localization
    Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.
  • Alternative names
    • R phycoerythrin alpha subunit antibody
    • R phycoerythrin beta subunit antibody
    • rpeA antibody
    • rpeB antibody

References

ab34730 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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