Anti-R Phycoerythrin antibody (HRP) (ab34727)

Overview

  • Product nameAnti-R Phycoerythrin antibody (HRP)
    See all R Phycoerythrin primary antibodies
  • Description
    Goat polyclonal to R Phycoerythrin (HRP)
  • ConjugationHRP
  • SpecificityThis antibody cross reacts with B Phycoerythrin.
  • Tested applicationsSuitable for: WB, Dot blot, ELISA, Electron Microscopymore details
  • Immunogen

    R Phycoerythrin from the seaweed Gracilaria.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • Purification notesThis antibody was prepared from monospecific antiserum by immunoaffinity chromatography using a R Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Dot blot
ELISA
Electron Microscopy
  • Application notesDot blot: Use at an assay dependent dilution.
    Immunoperoxidase Electron Microscopy: Use at an assay dependent dilution.
    ELISA: 1/2,000 - 1/10,000.
    WB: 1/500 - 1/2,000. Predicted molecular weight: 240 kDa.

    Not yet tested in other applications but thought to be suitable for other peroxidase-antibody based enzymatic assays.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevancePhycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
    • Cellular localizationPlastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.
    • Alternative names
      • R phycoerythrin alpha subunit antibody
      • R phycoerythrin beta subunit antibody
      • rpeA antibody
      • rpeB antibody

    References for Anti-R Phycoerythrin antibody (HRP) (ab34727)

    ab34727 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for sending this information.

    I've found the order information and looked up the lot number, so this is very helpfulfor our internal investigation into the problem with this antibody. You should be receiving the new vial of ab34727 ...

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    Thanks for your reply.

    Please take your time, it's no problem. Weare preparing new aliquots of the antibody and will be sending you another vial on the order ***. I expect this to arrive late next week.

    I apologize again for the inc...

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    Thank you for your reply and for keeping me udpated.

    I'm sorry for the delay, but all of our shipment-ready stock does contain the orange precipitate. I'm currently working with our supply chain to get some vials without this problem and hope...

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    Thanks for keeping me updated, and I apologize that there have been so many problems with this antibody.

    I am checking with the lab to see if there's another lotto send, and I will be happy to send a vial if so.

    I didn't realize whe...

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    Thank you for your call today and for sending the images, and I apologize for the trouble with this antibody.

    I just left a voicemail for you, but I wanted to be in touch via email as well. The scientists in the lab did say that they expect t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"