Overview

  • Product name
    Anti-R Phycoerythrin antibody (Rhodamine)
    See all R Phycoerythrin primary antibodies
  • Description
    Goat polyclonal to R Phycoerythrin (Rhodamine)
  • Conjugation
    Rhodamine. Ex: 550nm, Em: 570nm
  • Specificity
    This antibody recognizes R Phycoerythrin conjugated IgG. It will also cross react with B Phycoerythrin.
  • Tested applications
    Suitable for: Immunomicroscopy, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with the seaweed Gracilaria. Not yet tested in other species.
  • Immunogen

    Highly purified R Phycoerythrin from the seaweed Gracilaria.

  • General notes

    Label: Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons). Absorption Wavelength: 550 nm. Emission Wavelength: 570 nm. Fluorochrome/Protein Ratio: 4.0 moles TRITC per mole of Goat IgG.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was purified from monospecific antiserun by immunoaffinity chromatography using a R Phycoerythrin coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
Flow Cyt
WB
  • Application notes
    Flow Cyt: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 240 kDa. Reaction with low molecular fragments is typically noted by Western blot.

    This product is also suitable for other antibody based fluorescent assays.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
    • Cellular localization
      Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.
    • Alternative names
      • R phycoerythrin alpha subunit antibody
      • R phycoerythrin beta subunit antibody
      • rpeA antibody
      • rpeB antibody

    References for Anti-R Phycoerythrin antibody (Rhodamine) (ab34717)

    This product has been referenced in:
    • Fujita T  et al. Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP). Sci Rep 3:3171 (2013). Read more (PubMed: 24201379) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab34717.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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