Anti-R Phycoerythrin antibody (Texas Red) (ab34734)

Overview

  • Product nameAnti-R Phycoerythrin antibody (Texas Red)
    See all R Phycoerythrin primary antibodies
  • Description
    Goat polyclonal to R Phycoerythrin (Texas Red)
  • ConjugationTexas Red ®. Ex: 596nm, Em: 620nm
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and R-Phycoerythrin conjugated IgG. This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.
  • Tested applicationsSuitable for: Flow Cyt, Immunomicroscopymore details
  • Immunogen

    R Phycoerythrin from the seaweed gracilaria.

  • General notes

    Label: Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.5 moles Texas Red™ per mole of Goat IgG Conjugation Reference: J. Titus, P. Haugland, S. Sharrow, D. Segal J. Immunol. Methods 50; 193, 1982.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified by immunoaffinity chromatography using R Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2000 - 1/10000.
Immunomicroscopy 1/500 - 1/2500.

Target

  • RelevancePhycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
  • Cellular localizationPlastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.
  • Alternative names
    • R phycoerythrin alpha subunit antibody
    • R phycoerythrin beta subunit antibody
    • rpeA antibody
    • rpeB antibody

References for Anti-R Phycoerythrin antibody (Texas Red) (ab34734)

ab34734 has not yet been referenced specifically in any publications.

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R-PE is a variety of PE, (B-PE is another), so it is correct to say that R-PE is PE, but so is B-PE.

If you are trying to detect a PE conjugate, ab34734 is appropriate, as it will detect B-PE as well a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"