Anti-R Phycoerythrin antibody (Texas Red) (ab34734)

Overview

  • Product name
    Anti-R Phycoerythrin antibody (Texas Red)
    See all R Phycoerythrin primary antibodies
  • Description
    Goat polyclonal to R Phycoerythrin (Texas Red)
  • Conjugation
    Texas Red ®. Ex: 596nm, Em: 620nm
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and R-Phycoerythrin conjugated IgG. This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.
  • Tested applications
    Suitable for: Flow Cyt, Immunomicroscopymore details
  • Immunogen

    R Phycoerythrin from the seaweed gracilaria.

  • General notes

    Label: Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.5 moles Texas Red™ per mole of Goat IgG Conjugation Reference: J. Titus, P. Haugland, S. Sharrow, D. Segal J. Immunol. Methods 50; 193, 1982.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified by immunoaffinity chromatography using R Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2000 - 1/10000.
Immunomicroscopy 1/500 - 1/2500.

Target

  • Relevance
    Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
  • Cellular localization
    Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.
  • Alternative names
    • R phycoerythrin alpha subunit antibody
    • R phycoerythrin beta subunit antibody
    • rpeA antibody
    • rpeB antibody

References

ab34734 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us.

R-PE is a variety of PE, (B-PE is another), so it is correct to say that R-PE is PE, but so is B-PE.

If you are trying to detect a PE conjugate, ab34734 is appropriate, as it will detect B-PE as well a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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