Overview

  • Product name
  • Description
    Rabbit polyclonal to R2D2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Fruit fly (Drosophila melanogaster) R2D2.

    (Peptide available as ab17669.)

  • Positive control
    • This antibody gave a positive signal in Drosophila lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).

Target

  • Relevance
    The RNA interference (RNAi) pathway is initiated by processing long double-stranded RNA into small interfering RNA (siRNA). The siRNA-generating enzyme consists of DCR-2 and R2D2. R2D2 is homologous to the Caenorhabditis elegans RNAi protein RDE-4.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • CG7138 antibody
      • CG7138 PA antibody
      • LD06392p antibody

    Images

    • All lanes : Anti-R2D2 antibody (ab14750) at 1 µg/ml

      Lane 1 : Drosophila lysate
      Lane 2 : Drosophila lysate with Human R2D2 peptide (ab17669) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.


      Predicted band size : 35 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 17 kDa (possible cross reactivity),65 kDa (possible cross reactivity).

    References

    This product has been referenced in:
    • Gerbasi VR  et al. Proteomics identification of Drosophila small interfering RNA-associated factors. Mol Cell Proteomics 9:1866-72 (2010). WB ; Drosophila melanogaster . Read more (PubMed: 20472918) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab14750.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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