The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 60, 65 kDa (predicted molecular weight: 105 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay 1/62500.
RelevanceThe R3H motif is characterised by the presence of an invariant arginine residue and a highly conserved histidine residue, which are separated by three residues. The function of the domain is predicted to be binding ssDNA. There are two named isoforms of R3HDM2.