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Anti-RAB10 antibody [4E2] (ab104859)

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Overview

Product name

Anti-RAB10 antibody [4E2]
See all RAB10 products (3) ...

Description

Mouse monoclonal [4E2] to RAB10

Tested applications

ICC/IF, WB, ELISA, Flow Cytmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Purified recombinant fragment of Human RAB10 expressed in E. coli.

Positive control

Hela and NIH/3T3 cell lysates; LOVO cells

Properties

Form

Liquid

Storage instructions

Store at 4°C. Aliquot and store at -20°C long-term.

Storage buffer

Preservative: 0.03% Sodium Azide
Constituents: Ascites

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

4E2

Isotype

IgG1

Research areas

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family

  • Western blot - RAB10 antibody [4E2] (ab104859)Western blot - RAB10 antibody [4E2] (ab104859) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - RAB10 antibody [4E2] (ab104859)Immunocytochemistry/ Immunofluorescence - RAB10 antibody [4E2] (ab104859) image (enlarge)

  • Flow Cytometry - Anti-RAB10 antibody [4E2] (ab104859)Flow Cytometry - Anti-RAB10 antibody [4E2] (ab104859) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab104859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 1 Image

    ICC/IF

     ICC/IF: 1/1000.

    ICC/IF: 1/1000.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/2000. Pred...Read more →

    WB: 1/500 - 1/2000. Predicted molecular weight: 23 kDa.

  • ELISA

     ELISA: 1/10000.

    ELISA: 1/10000.

  • 1 Image

    Flow Cyt

     Flow Cyt: 1/100.

    Flow Cyt: 1/100.

Target

Function

May be involved in vesicular trafficking and neurotransmitter release.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Cellular localization

Cell membrane.

Target information above from: UniProt accessionP61026 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • GTP binding protein RAB10 antibody
  • Rab10 antibody
  • RAB10 member RAS oncogene family antibody
  • RAB10_HUMAN antibody
  • Ras related GTP binding protein antibody
  • Ras related GTP binding protein RAB10 antibody
  • Ras-related protein Rab-10 antibody
see all

Database links

see all

Anti-RAB10 antibody [4E2] images:

  Western blot - RAB10 antibody [4E2] (ab104859)

Western blot - RAB10 antibody [4E2] (ab104859)

All lanes : Anti-RAB10 antibody [4E2] (ab104859) at 1/500 dilution

Lane 1 : Hela cell lysate
Lane 2 : NIH/3T3 cell lysate


Predicted band size : 23 kDa

  Immunocytochemistry/ Immunofluorescence - RAB10 antibody [4E2] (ab104859)

Immunocytochemistry/ Immunofluorescence - RAB10 antibody [4E2] (ab104859)

Immunofluorescence analysis of LOVO cells using ab104859 (green). Blue: DRAQ5 fluorescent DNA dye.

  Flow Cytometry - Anti-RAB10 antibody [4E2] (ab104859)

Flow Cytometry - Anti-RAB10 antibody [4E2] (ab104859)

Overlay histogram showing HeLa cells stained with ab104859 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104859, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91653, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-RAB10 antibody [4E2] (ab104859)

ab104859 has not yet been referenced specifically in any publications.

Publishing research using ab104859? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"