- Datasheet
- Specific References (14)
- Protocols
Overview
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Product nameAnti-Rab11 antibody
See all Rab11 primary antibodies -
DescriptionRabbit polyclonal to Rab11
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, IPmore details
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Species reactivityReacts with: Dog, Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Pig, Non human primates
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Immunogen
Synthetic peptide corresponding to Human Rab11 aa 1-17.
Sequence:MGTRDDEYDYLFKVVLIC
(Peptide available asab41779) -
Positive control
- HeLa, PC3 or CV1 cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab3612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 18384686 | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human Rab11 peptide (ab41779). By Western blot, this antibody detects an ~22 kDa protein representing Rab 11 in CV1, PC3, and HeLa cell extract. Another less prominent, unknown band is also detected by this antibody at ~35 kDa. | |
| IP | Use at an assay dependent concentration. PubMed: 17686995 |
Target
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FunctionGTPase that modulates endosomal trafficking. Acts as a major regulator of membrane delivery during cytokinesis.
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Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
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Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 415544 Chicken
- Entrez Gene: 532723 Cow
- Entrez Gene: 403957 Dog
- Entrez Gene: 9230 Human
- Entrez Gene: 19326 Mouse
- Entrez Gene: 595117 Pig
- Entrez Gene: 100009072 Rabbit
- Entrez Gene: 79434 Rat
see all -
Alternative names
- GTP binding protein YPT3 antibody
- GTP-binding protein ypt3 antibody
- H-YPT3 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Rab11 antibody (ab3612)Image from Svendsen S et al., BMC Cell Biol. 2008 Apr 2;9:17. Fig 4.; doi:10.1186/1471-2121-9-17; 2 April 2008, BMC Cell Biology 2008, 9:17Immunofluorescence analysis of HeLa cells, staining Rab11 (red) with ab3612.
Cells were fixed with paraformaldehyde for 10 minutes, blocked in PBS with 5% normal goat serum and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/200). An AlexaFluor®594-conjugated anti-rabbit IgG (1/1000) was used as the secondary antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-Rab11 antibody (ab3612)This image is courtesy of an anonymous Abreviewab3612 staining HEK 293 cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.012% saponin prior to blocking in 1% BSA for 30 minutes at 37°C. The primary antibody was diluted 1/200 and incubated with the sample for 2 hours at 37°C. A biotinylated goat anti-rabbit antibody, diluted 1/200, was used as the secondary.
This image depicts ab3612 recognizing endogenous Rab11 (red). Rab11 was used as a recycling endosome marker.
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Western blot - Anti-Rab11 antibody (ab3612)ab3612 used on PC3 cell extract.
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Immunocytochemistry/ Immunofluorescence - Anti-Rab11 antibody (ab3612)Image courtesy of an anonymous Abreview.ab3612 staining Rab11 in Canine kidney cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.5% saponin, then incubated with ab3612 at a 1/200 dilution for 1.5 hours at 20°C. The secondary used was an Alexa-Fluor 568 conjugated donkey anti-rabbit polyclonal, used at a 1/350 dilution.
Protocols
References
This product has been referenced in:
- Del Cid-Pellitero E et al. Homeostatic Changes in GABA and Glutamate Receptors on Excitatory Cortical Neurons during Sleep Deprivation and Recovery. Front Syst Neurosci 11:17 (2017). Read more (PubMed: 28408870) »
- Kim SW et al. Covalent, Non-Covalent, Encapsulated Nanodrug Regulate the Fate of Intra- and Extracellular Trafficking: Impact on Cancer and Normal Cells. Sci Rep 7:6454 (2017). Read more (PubMed: 28743942) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"