Customer reviews and FAQs

Overview

  • Product name
    Anti-Rab11 antibody
    See all Rab11 primary antibodies
  • Description
    Rabbit polyclonal to Rab11
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Dog, Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Pig, Non human primates
  • Immunogen

    Synthetic peptide corresponding to Human Rab11 aa 1-17.
    Sequence:

    MGTRDDEYDYLFKVVLIC


    (Peptide available as ab41779)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • HeLa, PC3 or CV1 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab3612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 18384686
WB Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human Rab11 peptide (ab41779). By Western blot, this antibody detects an ~22 kDa protein representing Rab 11 in CV1, PC3, and HeLa cell extract. Another less prominent, unknown band is also detected by this antibody at ~35 kDa.
IP Use at an assay dependent concentration. PubMed: 17686995

Target

Anti-Rab11 antibody images

  • Immunocytochemistry/ Immunofluorescence - Anti-Rab11 antibody (ab3612)
    Immunocytochemistry/ Immunofluorescence - Anti-Rab11 antibody (ab3612)Image from Svendsen S et al., BMC Cell Biol. 2008 Apr 2;9:17. Fig 4.; doi:10.1186/1471-2121-9-17; 2 April 2008, BMC Cell Biology 2008, 9:17

    Immunofluorescence analysis of HeLa cells, staining Rab11 (red) with ab3612.
    Cells were fixed with paraformaldehyde for 10 minutes, blocked in PBS with 5% normal goat serum and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/200). An AlexaFluor®594-conjugated anti-rabbit IgG (1/1000) was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Rab11 antibody (ab3612)
    Immunocytochemistry/ Immunofluorescence - Rab11 antibody (ab3612)This image is courtesy of an anonymous Abreview

    ab3612 staining HEK 293 cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.012% saponin prior to blocking in 1% BSA for 30 minutes at 37°C.  The primary antibody was diluted 1/200 and incubated with the sample for 2 hours at 37°C. A biotinylated goat anti-rabbit antibody, diluted 1/200, was used as the secondary.

    This image depicts ab3612 recognizing endogenous Rab11 (red). Rab11 was used as a recycling endosome marker.

    See Abreview

  • Western blot - Rab11 antibody (ab3612)
    Western blot - Rab11 antibody (ab3612)


    Predicted band size : 24 kDa

    ab3612 used on PC3 cell extract.

  • Immunocytochemistry/ Immunofluorescence - Anti-Rab11 antibody (ab3612)
    Immunocytochemistry/ Immunofluorescence - Anti-Rab11 antibody (ab3612)Image courtesy of an anonymous Abreview.

    ab3612 staining Rab11 in Canine kidney cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.5% saponin, then incubated with ab3612 at a 1/200 dilution for 1.5 hours at 20°C. The secondary used was an Alexa-Fluor 568 conjugated donkey anti-rabbit polyclonal, used at a 1/350 dilution.

    See Abreview

References for Anti-Rab11 antibody (ab3612)

This product has been referenced in:
  • Kim JH  et al. Viral binding-induced signaling drives a unique and extended intracellular trafficking pattern during infection of primary monocytes. Proc Natl Acad Sci U S A 113:8819-24 (2016). Read more (PubMed: 27432979) »
  • Leksa V  et al. Dissecting Mannose 6-Phosphate-Insulin-like Growth Factor 2 Receptor Complexes That Control Activation and Uptake of Plasminogen in Cells. J Biol Chem 287:22450-62 (2012). WB . Read more (PubMed: 22613725) »

See all 9 Publications for this product

Publishing research using ab3612? Please let us know so that we can cite the reference in this datasheet.

Product Wall

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16 Abreviews or Q&A

10
Votes: Highest First

Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab11 antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hela)
Specification
hela
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Fixative
Paraformaldehyde
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Username

Dr. customer customer

Verified customer

Submitted May 09 2017

Western blot abreview for Anti-Rab11 antibody

 Good
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pacific oyster Cell lysate - whole cell (Gills proteome)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10%)
Loading amount
25 µg
Specification
Gills proteome
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Username

Abcam user community

Verified customer

Submitted Apr 28 2017

Western blot abreview for Anti-Rab11 antibody

 Average
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Hamster Cell lysate - whole cell (Baby hamster kidney fibroblast cells (BHK-21))
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Loading amount
10 µg
Specification
Baby hamster kidney fibroblast cells (BHK-21)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Username

Abcam user community

Verified customer

Submitted Mar 02 2016

IHC - Wholemount abreview for Anti-Rab11 antibody

 Inconclusive
Abreviews
Application
IHC - Wholemount
Sample
Fruit fly (Drosophila melanogaster) Tissue (Follicular epithelium)
Specification
Follicular epithelium
Read More
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Username

Mr. Veit Riechmann

Verified customer

Submitted May 14 2015


Inquiry: I am curious as to whether your Anti-Rab11 antibody is specific to Rab11A, Rab11B, or both?

Thank you for contacting us.

This antibody detects both RAB11A and Rab11B.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.


The cells I am using are HEK293 cells, so they are human. The protein concentration is unknown (I haven’t quantitated it), however, the same sample was used for Rab5 (ab18211, a marker for early endosomes) and beta-actin (ab8227), and those ...

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Thank you for your reply. I would recommend keeping the blocking to one hour and incubating the primary antibody overnight at 4 degrees. I would suggest doing this at 1/300 for ab74906 and at 1/1000 for ab3612. Please let me know if this does not impro...

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Inquiry: Dear Sir or Madam, I am currently looking for an anti-human Rab 11 antibody for immunocytochemistry to perform localization studies. Unfortunately, we have had negative experience with antibodies that were not specific or not sensitive enough ...

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Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guaran...

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Testing mouse samples in ICC.

Thank you for the further information. It is difficult to select alternatives that are the most 'specific' as we have not tested these antibodies side by side in the same sample application or species. Reviewing the datasheets I think that ab74906 a...

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I am going to titrate the monoclonal Rab11 antibody (ab3612) with my cell for IF. Would you suggest to series dilute the secondary antibody or keep its amount constant for different concentrations of primary antibody, when the secondary antibody is a...

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Thank you for contacting us. Yes, a suggested starting dilution for the primary antibody would be 1/200. And I would suggest keeping the secondary antibody dilution constant while titrating the primary antibody. Once you know the optimal dilution fo...

Read More

Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab11 antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (Kidney (ATCC CCL-34))
Specification
Kidney (ATCC CCL-34)
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% saponin
Read More
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Username

Abcam user community

Verified customer

Submitted Oct 07 2011

1-10 of 16 Abreviews or Q&A

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