Overview

  • Product nameAnti-Rab11 antibody
    See all Rab11 primary antibodies
  • Description
    Rabbit polyclonal to Rab11
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Dog, Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Pig, Non Human Primates
  • Immunogen

    Synthetic peptide corresponding to Human Rab11 aa 1-17.
    Sequence:

    MGTRDDEYDYLFKVVLIC


    (Peptide available as ab41779)

  • Positive control
    • HeLa, PC3 or CV1 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab3612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 18384686
WB Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human Rab11 peptide (ab41779). By Western blot, this antibody detects an ~22 kDa protein representing Rab 11 in CV1, PC3, and HeLa cell extract. Another less prominent, unknown band is also detected by this antibody at ~35 kDa.
IP Use at an assay dependent concentration. PubMed: 17686995

Target

Anti-Rab11 antibody images



  • Predicted band size : 24 kDa

    ab3612 used on PC3 cell extract.

  • ab3612 staining HEK 293 cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.012% saponin prior to blocking in 1% BSA for 30 minutes at 37°C.  The primary antibody was diluted 1/200 and incubated with the sample for 2 hours at 37°C. A biotinylated goat anti-rabbit antibody, diluted 1/200, was used as the secondary.

    This image depicts ab3612 recognizing endogenous Rab11 (red). Rab11 was used as a recycling endosome marker.

    See Abreview

  • ab3612 staining Rab11 in Canine kidney cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.5% saponin, then incubated with ab3612 at a 1/200 dilution for 1.5 hours at 20°C. The secondary used was an Alexa-Fluor 568 conjugated donkey anti-rabbit polyclonal, used at a 1/350 dilution.

    See Abreview

  • Immunofluorescence analysis of HeLa cells, staining Rab11 (red) with ab3612.
    Cells were fixed with paraformaldehyde for 10 minutes, blocked in PBS with 5% normal goat serum and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/200). An AlexaFluor®594-conjugated anti-rabbit IgG (1/1000) was used as the secondary antibody.

References for Anti-Rab11 antibody (ab3612)

This product has been referenced in:
  • Kim JH  et al. Viral binding-induced signaling drives a unique and extended intracellular trafficking pattern during infection of primary monocytes. Proc Natl Acad Sci U S A 113:8819-24 (2016). Read more (PubMed: 27432979) »
  • Leksa V  et al. Dissecting Mannose 6-Phosphate-Insulin-like Growth Factor 2 Receptor Complexes That Control Activation and Uptake of Plasminogen in Cells. J Biol Chem 287:22450-62 (2012). WB . Read more (PubMed: 22613725) »

See all 9 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Hamster Cell lysate - whole cell (Baby hamster kidney fibroblast cells (BHK-21))
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Loading amount 10 µg
Specification Baby hamster kidney fibroblast cells (BHK-21)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 02 2016

Application IHC - Wholemount
Sample Fruit fly (Drosophila melanogaster) Tissue (Follicular epithelium)
Specification Follicular epithelium
Username

Mr. VEIT Riechmann

Verified customer

Submitted May 14 2015

Thank you for contacting us.

This antibody detects both RAB11A and Rab11B.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your reply. I would recommend keeping the blocking to one hour and incubating the primary antibody overnight at 4 degrees. I would suggest doing this at 1/300 for ab74906 and at 1/1000 for ab3612. Please let me know if this does not impro...

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Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guaran...

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Thank you for the further information. It is difficult to select alternatives that are the most 'specific' as we have not tested these antibodies side by side in the same sample application or species. Reviewing the datasheets I think that ab74906 a...

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Thank you for contacting us. Yes, a suggested starting dilution for the primary antibody would be 1/200. And I would suggest keeping the secondary antibody dilution constant while titrating the primary antibody. Once you know the optimal dilution fo...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Dog Cell (Kidney (ATCC CCL-34))
Specification Kidney (ATCC CCL-34)
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% saponin
Username

Abcam user community

Verified customer

Submitted Oct 07 2011

Thank you for your reply. I can issue a discount code in retrospect, but we do ask that customers contact us before placing the initial order. The code will become valid once you have submitted the Abreview and can be used to purchase a second ...

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Thank you very much for your interest in ab3612. To our knowledge, ab3612 has not been tested in canine. Therefore, I can offer a discount off a future purchase if you buy ab3612 now, test it in canine and submit feedback to us in the form of a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"