• Product name
  • Description
    Rabbit polyclonal to RAB11-FIP2
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human RAB11-FIP2.

    (Peptide available as ab95920.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HEK293; HepG2; MCF7; JEG-3; A431.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab76892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 58 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • Function
    A Rab11 effector binding preferentially phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and phosphatidic acid (PA) and acting in the regulation of the transport of vesicles from the endosomal recycling compartment (ERC) to the plasma membrane. Involved in insulin granule exocytosis. Also involved in receptor-mediated endocytosis and membrane trafficking of recycling endosomes, probably originating from clathrin-coated vesicles. Required in a complex with MYO5B and RAB11 for the transport of NPC1L1 to the plasma membrane. Also acts as a regulator of cell polarity.
  • Sequence similarities
    Contains 1 C2 domain.
    Contains 1 FIP-RBD domain.
  • Post-translational
    Phosphorylation at Ser-227 by MARK2 regulates epithelial cell polarity.
  • Cellular localization
    Cell membrane. Recycling endosome membrane. Translocates with RAB11A from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0941 antibody
    • NRip11 antibody
    • RAB11 family interacting protein 2 (class I) antibody
    • Rab11 family-interacting protein 2 antibody
    • RAB11 FIP2 long isoform antibody
    • Rab11-FIP2 antibody
    • RAB11FIP2 antibody
    • RFIP2 antibody
    • RFIP2_HUMAN antibody
    see all


  • All lanes : Anti-RAB11-FIP2 antibody (ab76892) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 46 kDa (why is the actual band size different from the predicted?)

    Exposure time: 5 minutes

    The predicted molecular weight of RAB11-FIP2 is 58 kDa (Swissprot), but we are unsure of the reason why a reaction at 46 kDa is observed. Further characterization on this product is underway. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • ICC/IF image of ab76892 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76892, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


ab76892 has not yet been referenced specifically in any publications.

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