The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa). The observed molecular weight of 80kDa corresponds to earlier findings in literature with different antibodies (Lindsay et al, J Biol Chem. 2002 Apr 5;277(14):12190-9. PMID 11786538.).
FunctionA Rab11 effector protein involved in the endosomal recycling process. Also involved in controlling membrane trafficking along the phagocytic pathway and in phagocytosis.
Tissue specificityIsoform 2 is expressed in brain, heart, testis, lung, spleen, ovary and small intestine.
Cellular localizationRecycling endosome. Rab11A rather than Rab4A mediates localization in the endocytic recycling compartment (ERC) and Cytoplasmic vesicle, phagosome membrane. Membrane-bound (isoform 2). Colocalizes with Rab11A at phagosomes (isoform 2).