The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa). The observed molecular weight of 80kDa corresponds to earlier findings in literature with different antibodies (Lindsay et al, J Biol Chem. 2002 Apr 5;277(14):12190-9. PMID 11786538.).
A Rab11 effector protein involved in the endosomal recycling process. Also involved in controlling membrane trafficking along the phagocytic pathway and in phagocytosis.
Isoform 2 is expressed in brain, heart, testis, lung, spleen, ovary and small intestine.
Contains 1 C2 domain. Contains 1 FIP-RBD domain.
Recycling endosome. Rab11A rather than Rab4A mediates localization in the endocytic recycling compartment (ERC) and Cytoplasmic vesicle, phagosome membrane. Membrane-bound (isoform 2). Colocalizes with Rab11A at phagosomes (isoform 2).