Overview

  • Product nameAnti-RAB11FIP4 antibody
  • Description
    Rabbit polyclonal to RAB11FIP4
  • Specificityab74446 detects endogenous levels of total RAB11FIP4 protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal region of human RAB11FIP4.

  • Positive control
    • HepG2, 293, COLO, HUVEC and HT29 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab74446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 72 kDa.
ELISA 1/40000.

Target

  • RelevanceProteins of the large Rab GTPase family (see RAB1A; MIM 179508) have regulatory roles in the formation, targeting, and fusion of intracellular transport vesicles. RAB11FIP4 is one of many proteins that interact with and regulate Rab GTPases (Hales et al., 2001 [PubMed 11495908]). The RAB11FIP4 effector protein acts in endosomal trafficking.
  • Cellular localizationRecycling endosome membrane; Peripheral membrane protein. Note: Colocalizes with RAB11A in the endocytic recycling compartment (ERC).
  • Database links
  • Alternative names
    • Rab11 family interacting protein 4 antibody
    • RAB11 FIP4 antibody
    • Rab11 FIP4 like antibody
    • RGD1305063 antibody
    • RP23-271B20.4 antibody
    see all

Anti-RAB11FIP4 antibody images

  • All lanes : Anti-RAB11FIP4 antibody (ab74446) at 1/500 dilution

    Lane 1 : HepG2 cell extracts
    Lane 2 : 293 cell extracts
    Lane 3 : COLO cell extracts
    Lane 4 : HUVEC cell extracts
    Lane 5 : HT29 cell extracts
    Lane 6 : 293 cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 72 kDa
    Observed band size : 72 kDa
    Additional bands at : 20 kDa,40 kDa. We are unsure as to the identity of these extra bands.

References for Anti-RAB11FIP4 antibody (ab74446)

ab74446 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Zebra finch Tissue sections (Zebra finch Brain, 30um sections)
Specification Zebra finch Brain, 30um sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 0.3% TBS/Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Jul 09 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"