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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab74446 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 72 kDa.
Relevance Proteins of the large Rab GTPase family (see RAB1A; MIM 179508) have regulatory roles in the formation, targeting, and fusion of intracellular transport vesicles. RAB11FIP4 is one of many proteins that interact with and regulate Rab GTPases (Hales et al., 2001 [PubMed 11495908]). The RAB11FIP4 effector protein acts in endosomal trafficking.
Cellular localization Recycling endosome membrane; Peripheral membrane protein. Note: Colocalizes with RAB11A in the endocytic recycling compartment (ERC).
Rab11 family interacting protein 4 antibody
RAB11 FIP4 antibody
Rab11 FIP4 like antibody
Anti-RAB11FIP4 antibody images
Western blot - RAB11FIP4 antibody (ab74446)
All lanes : Anti-RAB11FIP4 antibody (ab74446) at 1/500 dilution Lane 1 : HepG2 cell extracts Lane 2 : 293 cell extracts Lane 3 : COLO cell extracts Lane 4 : HUVEC cell extracts Lane 5 : HT29 cell extracts Lane 6 : 293 cell extracts with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 72 kDa Observed band size : 72 kDa Additional bands at : 20 kDa,40 kDa. We are unsure as to the identity of these extra bands.
References for Anti-RAB11FIP4 antibody (ab74446)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"