Overview

  • Product name
  • Description
    Mouse polyclonal to RAB11FIP5
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-652 of Human RAB11FIP5

  • Positive control
    • RAB11FIP5 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab68947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 70 kDa).

Target

  • Function
    Rab effector involved in protein trafficking from apical recycling endosomes to the apical plasma membrane.
  • Tissue specificity
    Detected at low levels in heart, brain, placenta, lung, liver, adipocytes, kidney, spleen, skeletal muscle and pancreas.
  • Sequence similarities
    Contains 1 C2 domain.
    Contains 1 FIP-RBD domain.
  • Domain
    Binds to vesicles enriched in neutral phospholipids via its C2 domain. The interaction is favored by Mg(2+) rather than Ca(2+).
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues.
  • Cellular localization
    Cytoplasm. Recycling endosome membrane. Mitochondrion membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GAF 1 antibody
    • Gaf-1 antibody
    • GAF1 antibody
    • gamma SNAP associated factor 1 antibody
    • Gamma-SNAP-associated factor 1 antibody
    • KIAA0857 antibody
    • Phosphoprotein pp75 antibody
    • pp75 antibody
    • RAB11 family interacting protein 5 (class I) antibody
    • Rab11 family-interacting protein 5 antibody
    • Rab11 FIP5 antibody
    • Rab11 interacting protein antibody
    • Rab11 interacting protein Rip11 antibody
    • Rab11-FIP5 antibody
    • Rab11-interacting protein Rip11 antibody
    • Rab11fip5 antibody
    • RFIP5_HUMAN antibody
    • RIP11 antibody
    see all

Images

  • All lanes : Anti-RAB11FIP5 antibody (ab68947) at 1/500 dilution

    Lane 1 : RAB11FIP5 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 70 kDa
    Observed band size : 77 kDa (why is the actual band size different from the predicted?)

References

ab68947 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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