The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
Use at an assay dependent dilution.
FunctionCould participate in polarized transport, in the assembly and/or the activity of tight junctions.
Tissue specificityDetected in several types of epithelia, including intestine, kidney, liver and in endothelial cells.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
Cellular localizationCell junction > tight junction. Cell membrane. Cytoplasmic vesicle membrane. Cytoplasmic tight junctions or associated with vesicles scattered throughout the cytoplasm in cells lacking tight junctions.
Almeida MQ et al. Activation of cyclic AMP signaling leads to different pathway alterations in lesions of the adrenal cortex caused by germline PRKAR1A defects versus those due to somatic GNAS mutations. J Clin Endocrinol Metab97:E687-93 (2012).
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