Overview

  • Product name
  • Description
    Rabbit polyclonal to RAB14
  • Tested applications
    Suitable for: ICC/IF, ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide derived from a region between amino acids 165-214 of human RAB14. (Note: the exact amino acid sequence is proprietary).

  • Positive control
    • Raji cell lysate, Human kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab28639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/12500.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 24 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-RAB14 antibody (ab28639) at 0.5 µg/ml + Raji cell lysate 10ug.

    Secondary
    HRP conjugated anti-Rabbit IgG

    Predicted band size : 24 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
  • Human kidney tissue stained with ab28639 at X400 magnigication. The arrows point to epithelial cells of the renal tubule [positive label].
  • ICC/IF image of ab28639 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28639, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-RAB14 antibody (ab28639) at 2 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with blocking peptide at 2

    Lysates/proteins at 2 µg/ml per lane.


    Predicted band size : 24 kDa

References

This product has been referenced in:
  • Reed SE  et al. A role for Rab14 in the endocytic trafficking of GLUT4 in 3T3-L1 adipocytes. J Cell Sci 126:1931-41 (2013). Mouse . Read more (PubMed: 23444368) »
  • Fonsato V  et al. Human liver stem cell-derived microvesicles inhibit hepatoma growth in SCID mice by delivering antitumor microRNAs. Stem Cells 30:1985-98 (2012). IF . Read more (PubMed: 22736596) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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