ICC/IF image of ab28639 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28639, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-RAB14 antibody (ab28639)
All lanes : Anti-RAB14 antibody (ab28639) at 2 µg/ml
Lane 1 : HepG2 cell lysate Lane 2 : HepG2 cell lysate with blocking peptide at 2
Lysates/proteins at 2 µg/ml per lane.
Predicted band size : 24 kDa
References for Anti-RAB14 antibody (ab28639)
This product has been referenced in:
Reed SE et al. A role for Rab14 in the endocytic trafficking of GLUT4 in 3T3-L1 adipocytes. J Cell Sci126:1931-41 (2013).
Read more (PubMed: 23444368) »
Mukhopadhyay A et al. Proteomic analysis of endocytic vesicles: Rab1a regulates motility of early endocytic vesicles. J Cell Sci124:765-75 (2011).
Read more (PubMed: 21303926) »