Overview

  • Product nameAnti-RAB14 antibody
    See all RAB14 primary antibodies
  • Description
    Rabbit polyclonal to RAB14
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Pig, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human RAB14.

    (Peptide available as ab41804.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Brain (Human), Brain (Mouse), Brain (Rat) and Raji (data not shown).

Properties

Applications

Our Abpromise guarantee covers the use of ab40938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 24 kDa).

Target

Anti-RAB14 antibody images

  • All lanes : Anti-RAB14 antibody (ab40938) at 1 mg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution

    Performed under non-reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 24 kDa


    Exposure time : 8 minutes
  • All lanes : Anti-RAB14 antibody (ab40938) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Mouse Tissue Lysate
    Lane 3 : Brain (Rat) Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)
    The RAB14 protein has several Ser/Thr residues which may be phosphorylated (SwissProt data). This could cause the doublet observed at the expected molecular weight.
  • ab40938 staining RAB14 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. HeLa cells were infected with C. trachomatis serovar L2 (MOI 5) for 10 hours. The cells were fixed in paraformaldehyde, permeabilised with saponin and then blocked using 0.2% BSA for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/100 for 1 hour at 25°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to FITC (green) used at a 1/200 dilution. Microtubules were evinced by a mouse monoclonal anti-ß-tubulin (1/200) followed by donkey anti-mouse Cy5-labeled antibodies (1/700) (red). Endogenous Rab14 was recruited to the chlamydial inclusion (blue asterisk). Bacteria were labeled with Hoechst 0.1 mg/ml (blue). ab40938 was useful to detect for the first time the recruitment of an endogenous RAB to the chlamydial inclusion.

    See Abreview

References for Anti-RAB14 antibody (ab40938)

This product has been referenced in:
  • Comellas AP  et al. Insulin regulates alveolar epithelial function by inducing Na+/K+-ATPase translocation to the plasma membrane in a process mediated by the action of Akt. J Cell Sci 123:1343-51 (2010). WB ; Human . Read more (PubMed: 20332111) »
  • Capmany A & Damiani MT Chlamydia trachomatis intercepts Golgi-derived sphingolipids through a Rab14-mediated transport required for bacterial development and replication. PLoS One 5:e14084 (2010). ICC/IF ; Human . Read more (PubMed: 21124879) »

See all 2 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells)
Specification HeLa cells
Fixative Paraformaldehyde
Permeabilization Yes - saponin
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Username

Dr. María Damiani

Verified customer

Submitted Dec 31 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"