Overview

  • Product name
  • Description
    Rabbit polyclonal to Rab17
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-210 of Human Rab17 (Q9H0T7).

  • Positive control
    • NT2D1, IMR32, U87-MG and MCF7 whole cell lysates, MCF7 cells, H661 xenograft

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 23 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Might be involved in transcellular transport.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AW413472 antibody
    • FLJ12538 antibody
    • OTTHUMP00000164417 antibody
    • OTTHUMP00000202805 antibody
    • Rab 17 antibody
    • Rab17 antibody
    • RAB17 member RAS oncogene family antibody
    • RAB17_HUMAN antibody
    • Ras related protein Rab 17 antibody
    • Ras related protein Rab17 antibody
    • Ras-related protein Rab-17 antibody
    see all

Images

  • All lanes : Anti-Rab17 antibody (ab155135) at 1/2000 dilution

    Lane 1 : U87-MG whole cell lysate
    Lane 2 : MCF7 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 23 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed MCF7 cells labeling Rab17 with ab155135 at 1/200. Image at the bottom costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin embedded H661 xenograft labeling Rab17 with ab155135 antibody at 1/500.

References

ab155135 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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