• Product nameAnti-Rab1A antibody
    See all Rab1A primary antibodies
  • Description
    Rabbit polyclonal to Rab1A
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 155 - 204 (AKNATNVEQS FMTMAAEIKK RMGPGATAGG AEKSNVKIQS TPVKQSGGGC) of Human Rab1A (NP_004152).

  • Positive control
    • Fetal muscle lysate



Our Abpromise guarantee covers the use of ab97956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.


  • FunctionThe small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion. Regulates the level of CASR present at the cell membrane. Plays a role in cell adhesion and cell migration, via its role in protein trafficking. Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Plays a role in microtubule-dependent protein transport by early endosomes and in anterograde melanosome transport.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
  • Post-translational
    Phosphorylated by CDK1 kinase during mitosis.
    Phosphocholinated at Ser-79 by L.pneumophila AnkX, leading to displace GDP dissociation inhibitors (GDI). Both GDP-bound and GTP-bound forms can be phosphocholinated. Dephosphocholinated by L.pneumophila Lem3, restoring accessibility to L.pneumophila GTPase effector LepB.
  • Cellular localizationGolgi apparatus. Endoplasmic reticulum. Early endosome. Cytoplasm, cytosol. Membrane. Melanosome. Alternates between membrane-associated and cytosolic forms.
  • Information by UniProt
  • Database links
  • Alternative names
    • GTP binding protein RAB 1A antibody
    • mKIAA3012 antibody
    • RAB 1 antibody
    • Rab 1A antibody
    • RAB1 antibody
    • RAB1, member RAS oncogene family antibody
    • Rab1A antibody
    • RAB1A member RAS oncogene family antibody
    • RAB1A_HUMAN antibody
    • Ras related protein Rab 1A antibody
    • Ras-associated protein RAB1 antibody
    • Ras-related protein Rab-1A antibody
    • YPT1 antibody
    • YPT1 related protein antibody
    • YPT1-related protein antibody
    see all

Anti-Rab1A antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling Rab1A with ab97956 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Rab1A. Right - Merge.
  • Anti-Rab1A antibody (ab97956) at 1/2000 dilution + Human Panc-1 whole cell lysate at 35 µg

    HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Armen Petrosyan

    See Abreview

  • All lanes : Anti-Rab1A antibody (ab97956) at 2 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with blocking peptide at 5 µg/ml

    Lysates/proteins at 25 µg per lane.

    Predicted band size : 23 kDa
  • Anti-Rab1A antibody (ab97956) at 1 µg/ml + Human fetal muscle lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 23 kDa
    Gel concentration: 12%

References for Anti-Rab1A antibody (ab97956)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 35 µg
Gel Running Conditions Reduced Denaturing (15% SDS-PAGE)
Sample Human Cell lysate - whole cell (Panc-1 cells)
Specification Panc-1 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Nov 13 2013

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample Human Cell (LNCaPcells)
Specification LNCaPcells
Permeabilization No
Fixative Paraformaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Nov 13 2013