Overview

  • Product nameAnti-RAB1B antibody
    See all RAB1B primary antibodies
  • Description
    Rabbit polyclonal to RAB1B
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 165-194 of Human RAB1B (NP_112243.1).

  • Positive control
    • 293 cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab116336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 22 kDa.

Target

  • FunctionProtein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
  • Post-translational
    modifications
    Prenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1).
    AMPylation at Tyr-77 occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB1B constitutively active.
  • Cellular localizationMembrane. Cytoplasm. Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments. In the GDP-form, colocalizes with GDI in the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • RAB1B antibody
    • RAB1B member RAS oncogene family antibody
    • RAB1B_HUMAN antibody
    • Ras related protein Rab1B antibody
    • Ras-related protein Rab-1B antibody
    • Small GTP binding protein antibody
    see all

Anti-RAB1B antibody images

  • Anti-RAB1B antibody (ab116336) at 1/100 dilution + 293 cell line lysate at 35 µg

    Predicted band size : 22 kDa

References for Anti-RAB1B antibody (ab116336)

ab116336 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Sample Mouse Cell (RAW264.7 Murine Macrophage Cell)
Specification RAW264.7 Murine Macrophage Cell
Permeabilization Yes - 0.5% Tween 20 for 1hr @ Roomtemp
Fixative Paraformaldehyde 2.5% pH 7.4
Username

Mr. Michael Connor

Verified customer

Submitted Sep 18 2014

Application Western blot
Loading amount 1e+006 cells
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris, MOPS running buffer)
Sample Human Cell lysate - whole cell (293T)
Specification 293T
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Constantin Takacs

Verified customer

Submitted Nov 19 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"