Overview

  • Product nameAnti-RAB1B antibody
    See all RAB1B primary antibodies
  • Description
    Mouse monoclonal to RAB1B
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 91 - 202 of human RAB1B protein (NP_112243) with a tag

  • Positive control
    • A-431 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab76721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 22 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionProtein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.
    • Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
    • Post-translational
      modifications
      Prenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1).
      AMPylation at Tyr-77 occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB1B constitutively active.
    • Cellular localizationMembrane. Cytoplasm. Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments. In the GDP-form, colocalizes with GDI in the cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • RAB1B antibody
      • RAB1B member RAS oncogene family antibody
      • RAB1B_HUMAN antibody
      • Ras related protein Rab1B antibody
      • Ras-related protein Rab-1B antibody
      • Small GTP binding protein antibody
      see all

    Anti-RAB1B antibody images

    • Anti-RAB1B antibody (ab76721) at 5 µg/ml + immunogen at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 22 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    • Anti-RAB1B antibody (ab76721) at 5 µg/ml + A431 cell lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 22 kDa
      Observed band size : 22 kDa

    References for Anti-RAB1B antibody (ab76721)

    ab76721 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76721.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"