The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionRequired for protein transport from the endoplasmic reticulum to the Golgi complex.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
Cellular localizationEndoplasmic reticulum-Golgi intermediate compartment membrane. Melanosome. Endoplasmic reticulum membrane. Golgi apparatus membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.