Overview

  • Product nameAnti-Rab20 antibody
    See all Rab20 primary antibodies
  • Description
    Rabbit polyclonal to Rab20
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 49- 200 of Human Rab20 (AAH26025).

  • Positive control
    • WB: HeLa, HepG2 and HCT116 cell lysate IF: HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab105043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 26 kDa.

Target

  • FunctionPlays a role in apical endocytosis/recycling. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes.
  • Tissue specificityLow or absent expression in normal pancreas and stronger expression in 15 of 18 exocrine pancreatic adenocarcinomas (at protein level).
  • Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
  • Cellular localizationGolgi apparatus. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle, phagosome membrane. Highly enriched on apical endocytic structures in polarized epithelial cells of kidney proximal tubules (By similarity). Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211).
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ20429 antibody
    • RAB20 antibody
    • RAB20 member RAS oncogene family antibody
    • RAB20_HUMAN antibody
    • Ras related protein Rab20 antibody
    • Ras-related protein Rab-20 antibody
    see all

Anti-Rab20 antibody images

  • Anti-Rab20 antibody (ab105043) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 26 kDa
  • ab105043, at a 1/200 dilution, staining Rab20 in methanol-fixed HeLa cells. Right image is costained with Hoechst 33342.

References for Anti-Rab20 antibody (ab105043)

ab105043 has not yet been referenced specifically in any publications.

Product Wall

The immunogen that was used to generate ab105043 shows 81% identity with rat Rab20, so there is a possibility that it will cross-react.

I hope this is helpful. Please let me know if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"