• Product name
  • Description
    Rabbit polyclonal to RAB21
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human RAB21.

    (Peptide available as ab89375.)

  • Positive control
    • This antibody gave a positive signal in Human liver tissue lysate and in the following whole cell lysates: HepG2; HeLa; A431; Jurkat.



Our Abpromise guarantee covers the use of ab79713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).


  • Function
    Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general. As a result, may regulate cell adhesion and migration (By similarity). During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis.
  • Tissue specificity
    Widely expressed. In jejunal tissue, predominantly expressed in the apical region of the epithelial cell layer of the villi, weak expression, if any, in the crypt epithelium. Capillary endothelium and some cell types in the lamina propria also show expression.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization
    Endoplasmic reticulum membrane. Golgi apparatus membrane. Early endosome membrane. Cytoplasmic vesicle membrane. Cleavage furrow. In nonpolarized epithelial Caco-2 cells, found in the endoplasmic reticulum; in polarized cells, observed in vesicles in the apical cytoplasm. During mitosis, in mid-telophase, localized in the ingressing cleavage furrow. In late telophase, detected at the opposite poles of the daughter cells, in vesicles at the base of lamellipodia formed by the separating daughter cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0118 antibody
    • RAB 21 antibody
    • RAB21 antibody
    • RAB21, member RAS oncogene family antibody
    • RAB21_HUMAN antibody
    • Ras-related protein Rab-21 antibody
    see all

Anti-RAB21 antibody images

  • All lanes : Anti-RAB21 antibody (ab79713) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 12.5 kDa,146 kDa,38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

References for Anti-RAB21 antibody (ab79713)

ab79713 has not yet been referenced specifically in any publications.

Product Wall

Western blot
Human Cell lysate - whole cell (HAEC-human arterial endothelial cells)
Loading amount
30 µg
HAEC-human arterial endothelial cells
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 45°C

Mrs. Elena Astanina

Verified customer

Submitted Jun 01 2011


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