Overview

  • Product nameAnti-RAB21 antibody
    See all RAB21 primary antibodies
  • Description
    Rabbit polyclonal to RAB21
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human RAB21.

    (Peptide available as ab89377.)

  • Positive control
    • This antibody gave a positive signal in Human liver tissue lysate and in the following whole cell lysates: HepG2; HeLa; A431; Jurkat.

Properties

Applications

Our Abpromise guarantee covers the use of ab88762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRegulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general. As a result, may regulate cell adhesion and migration (By similarity). During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis.
    • Tissue specificityWidely expressed. In jejunal tissue, predominantly expressed in the apical region of the epithelial cell layer of the villi, weak expression, if any, in the crypt epithelium. Capillary endothelium and some cell types in the lamina propria also show expression.
    • Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
    • Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane. Early endosome membrane. Cytoplasmic vesicle membrane. Cleavage furrow. In nonpolarized epithelial Caco-2 cells, found in the endoplasmic reticulum; in polarized cells, observed in vesicles in the apical cytoplasm. During mitosis, in mid-telophase, localized in the ingressing cleavage furrow. In late telophase, detected at the opposite poles of the daughter cells, in vesicles at the base of lamellipodia formed by the separating daughter cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA0118 antibody
      • RAB 21 antibody
      • RAB21 antibody
      • RAB21, member RAS oncogene family antibody
      • RAB21_HUMAN antibody
      • Ras-related protein Rab-21 antibody
      see all

    Anti-RAB21 antibody images

    • All lanes : Anti-RAB21 antibody (ab88762) at 1 µg/ml

      Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 5 : Human liver tissue lysate - total protein (ab29889)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 24 kDa
      Observed band size : 26 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 12.5 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 4 minutes

    References for Anti-RAB21 antibody (ab88762)

    ab88762 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HAEC-human arterial endothelial cells)
    Loading amount 30 µg
    Specification HAEC-human arterial endothelial cells
    Gel Running Conditions Reduced Denaturing (4-15% gradient)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 45°C
    Username

    Mrs. Elena Astanina

    Verified customer

    Submitted May 12 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"