Overview

  • Product nameAnti-Rab25 antibody [3F12]
    See all Rab25 primary antibodies
  • Description
    Mouse monoclonal [3F12] to Rab25
  • Tested applicationsSuitable for: ELISA, WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment from Rab25, expressed in E. coli.

  • Positive control
    • WB: MCF7, T47D and GC7901 cell lysates IHC-P: Human esophagus and lung cancer tissues ICC/IF/Flow cytometry: NIH 3T3 cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab106175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/2000. Predicted molecular weight: 23 kDa.
IHC-P 1/200 - 1/1000.
ICC/IF 1/200 - 1/1000.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionInvolved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia (PubMed:17925226). Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions (By similarity). May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis.
  • Tissue specificityExpressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer; expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancers. Expressed in breast cancer; expression is increased relative to normal breast tissue.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
  • Cellular localizationCell membrane. Cell projection, pseudopodium membrane. Cytoplasmic vesicle. Colocalizes with integrin alpha-V/beta-1 in vesicles at the pseudopodial tips.
  • Information by UniProt
  • Database links
  • Alternative names
    • CATX 8 antibody
    • CATX-8 antibody
    • CATX8 antibody
    • RAB11C antibody
    • RAB25 antibody
    • RAB25 member RAS oncogene family antibody
    • RAB25_HUMAN antibody
    • Ras related protein Rab 25 antibody
    • Ras-related protein Rab-25 antibody
    • RP11-336K24.10-001 antibody
    see all

Anti-Rab25 antibody [3F12] images

  • All lanes : Anti-Rab25 antibody [3F12] (ab106175) at 1/500 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : T47D cell lysate
    Lane 3 : GC7901 cell lysate


    Predicted band size : 23 kDa
  • Immunohistochemical analysis of Rab25 in paraffin embedded esophagus tissue, using ab106175 at a 1/200 dilution.
  • Immunohistochemical analysis of Rab25 in paraffin embedded Human lung cancer tissue, using ab106175 at a 1/200 dilution.
  • Flow cytometric analysis of NIH 3T3 cells using ab106175 (green) and a negative control (purple).

References for Anti-Rab25 antibody [3F12] (ab106175)

ab106175 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"