Overview

  • Product nameAnti-RAB27A antibody
    See all RAB27A primary antibodies
  • Description
    Mouse monoclonal to RAB27A
  • Tested applicationsSuitable for: WB, IHC-P, IP, IHC-FoFr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit
  • Immunogen

    Recombinant fragment: YCENPDIVLC GNKSDLEDQR VVKEEEAIAL AEKYGIPYFE TSAANGTNIS QAIEMLLDLI MKRMERCVDK SWIPEGVVRS NGHASTDQLS EEKEKGACGC , corresponding to amino acids 122-222 of Human RAB27A

Properties

Applications

Our Abpromise guarantee covers the use of ab55667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 25 kDa.
IHC-P Use a concentration of 3 µg/ml.
IP Use at an assay dependent concentration. See Abreview.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21832089
ICC/IF 1/200.

Target

  • FunctionPlays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
  • Tissue specificityFound in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.
  • Involvement in diseaseDefects in RAB27A are a cause of Griscelli syndrome type 2 (GS2) [MIM:607624]. Griscelli syndrome is a rare autosomal recessive disorder that results in pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes. GS2 patients also develop an uncontrolled T-lymphocyte and macrophage activation syndrome, known as hemophagocytic syndrome, leading to death in the absence of bone marrow transplantation. Neurological impairment is present in some patients, likely as a result of hemophagocytic syndrome.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
  • Cellular localizationMembrane. Melanosome. Late endosome. Lysosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes to endosomal exocytic vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • GS2 antibody
    • GTP-binding protein Ram antibody
    • HsT18676 antibody
    • MGC117246 antibody
    • Mutant Ras related protein Rab-27A antibody
    • Rab-27 antibody
    • RAB-27A antibody
    • RAB27 antibody
    • RAB27A antibody
    • RAB27A member RAS oncogene family antibody
    • RAM antibody
    • Ras-related protein Rab-27A antibody
    • Ras-related protein Rab27A antibody
    • RB27A_HUMAN antibody
    see all

Anti-RAB27A antibody images

  • RAB27A antibody (ab55667) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymphoma. Heat induced antigen retrieval with Citrate buffer pH 6 has been performed prior to immunostaining.
  • ab55667 staining cow Retinal Pigment Epithelium (RPE) cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 20 minutes at 25°C.  The primary antibody was diluted 1/200 and incubated with the sample for 12 hours at 4°C.  An Alexa Fluor® 546 conjugated goat anti-mouse antibody was used as the secondary.  Rab27a, which localizes to the apical site of the RPE cells, is labeled with red fluorescence. Nuclei were counterstained with DAPI (blue).

    See Abreview

  • All lanes : Anti-RAB27A antibody (ab55667) at 1/200 dilution

    Lane 1 : whole cell lysate of HEK 293 transfected with empty pCDNA3 vector
    Lane 2 : whole cell lysate of HEK 293 cells expressing Rab27a-GFP

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated goat anti-mouse
    Developed using the ECL technique

    Predicted band size : 25 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 minutes

    This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic

    See Abreview

  • ab55667 (undiluted) was incubated with whole cell lysate of HEK 293 cells transfected with Rab27a-GFP and a Protein G matrix for 12 hours at 4°C to achieve immunoprecipitation.  20µg of lysate were present in the input.

    ab55667 (diluted 1/200) was also used in the western blotting step.

    Lane 1. lysate of HEK 293 cells expressing Rab27a-GFP
    Lane 2. IP with ab55667
    Lane 3. Not bound fraction

    See Abreview

References for Anti-RAB27A antibody (ab55667)

This product has been referenced in:
  • Heinonen MT  et al. Tubulin- and actin-associating GIMAP4 is required for IFN-? secretion during Th cell differentiation. Immunol Cell Biol 93:158-66 (2015). IF ; Human . Read more (PubMed: 25287446) »
  • Wang Q  et al. High expression of RAB27A and TP53 in pancreatic cancer predicts poor survival. Med Oncol 32:372 (2015). IHC-P ; Human . Read more (PubMed: 25428385) »

See all 10 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rabbit Tissue sections (intestinum)
Specification intestinum
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step Blocking Solution Zytomed as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Mar 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (kidney)
Specification kidney
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization No
Blocking step (agent) for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Dec 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (SK-MEL-28)
Specification SK-MEL-28
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X in PBS
Username

Abcam user community

Verified customer

Submitted Oct 18 2012

Selon nos informations vous avez connu quelques difficultés avec ab55667 et avez contacté notre service scientifique (en février).

Nous vous avons donné quelques suggestions pour améliorer le protocole. J...

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Merci d'avoir pris le temps de remplir notre questionnaire et de nous avoir contactés. Je suis désolée d'apprendre que vous avez des difficultés à obtenir de bons résultats avec ab55667.

Merci d'avoir patienté pendant que je discutais avec l...

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Merci pour votre appel. Je suis désolée d'apprendre que ab55667 ne vous donne pas de bons résultats en immunohistochimie.

Veuillez trouver ci-joint un questionnaire, que nous avons discuté, qui nous permet de regrouper le plus d'informations...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HEK 293 cells transfected with hRab27a-GFP)
Total protein in input 20 µg
Specification HEK 293 cells transfected with hRab27a-GFP
Immuno-precipitation step Protein G
Username

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Feb 29 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK-293 cells transfected with human Rab27a-GFP)
Loading amount 20 µg
Specification HEK-293 cells transfected with human Rab27a-GFP
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Feb 29 2008

Application Immunocytochemistry/ Immunofluorescence
Sample Cow Cell (Retinal Pigment Epithelium cells)
Specification Retinal Pigment Epithelium cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.5%TX-100
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Feb 21 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cultured Cells (HEK-293 cells transfected with human Rab27a)
Specification HEK-293 cells transfected with human Rab27a
Fixative Paraformaldehyde
Permeabilization Yes - 0,5% TX100
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Feb 21 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"