Overview

  • Product name
  • Description
    Mouse polyclonal to RAB27B
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human RAB27B (AAH27474)

  • Positive control
    • RAB27B transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab76779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Target

  • Function
    May be involved in targeting uroplakins to urothelial apical membranes.
  • Tissue specificity
    Expressed primarily in testis.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C25KG antibody
    • Rab27b antibody
    • RAB27B member RAS oncogene family antibody
    • RAS associated protein RAB27B antibody
    • Ras related protein Rab 27b antibody
    • Ras related protein Rab27b antibody
    • Ras-related protein Rab-27B antibody
    • RB27B_HUMAN antibody
    • Small GTP binding protein rab27b antibody
    see all

Anti-RAB27B antibody images

  • All lanes : Anti-RAB27B antibody (ab76779) at 1/500 dilution

    Lane 1 : RAB27B transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 25 kDa
    Observed band size : 25 kDa

References for Anti-RAB27B antibody (ab76779)

This product has been referenced in:
  • McGrath JA  et al. Germline Mutation in EXPH5 Implicates the Rab27B Effector Protein Slac2-b in Inherited Skin Fragility. Am J Hum Genet 91:1115-21 (2012). Read more (PubMed: 23176819) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa Cells)
Loading amount
20 µg
Specification
HeLa Cells
Gel Running Conditions
Non-reduced Denaturing (12.5% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

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Submitted Jun 08 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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