Anti-RAB27B antibody (ab76779)
Key features and details
- Mouse polyclonal to RAB27B
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RAB27B antibody
See all RAB27B primary antibodies -
Description
Mouse polyclonal to RAB27B -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human RAB27B. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- RAB27B transfected 293T cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
Target
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Function
May be involved in targeting uroplakins to urothelial apical membranes. -
Tissue specificity
Expressed primarily in testis. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5874 Human
- Omim: 603869 Human
- SwissProt: O00194 Human
- Unigene: 25318 Human
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Alternative names
- C25KG antibody
- Rab27b antibody
- RAB27B member RAS oncogene family antibody
see all
Images
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All lanes : Anti-RAB27B antibody (ab76779) at 1/500 dilution
Lane 1 : RAB27B transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Datasheets and documents
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Datasheet download
References (5)
ab76779 has been referenced in 5 publications.
- Park SS et al. Temsirolimus Enhances Anti-Cancer Immunity by Inducing Autophagy-Mediated Degradation of the Secretion of Small Extracellular Vesicle PD-L1. Cancers (Basel) 14:N/A (2022). PubMed: 36077620
- Zhou H et al. Therapeutic inhibition of GAS6-AS1/YBX1/MYC axis suppresses cell propagation and disease progression of acute myeloid leukemia. J Exp Clin Cancer Res 40:353 (2021). PubMed: 34753494
- Underwood R et al. The GTPase Rab27b regulates the release, autophagic clearance, and toxicity of a-synuclein. J Biol Chem 295:8005-8016 (2020). PubMed: 32350025
- Stephens DC et al. Spatiotemporal organization and protein dynamics involved in regulated exocytosis of MMP-9 in breast cancer cells. J Gen Physiol 151:1386-1403 (2019). PubMed: 31676484
- McGrath JA et al. Germline Mutation in EXPH5 Implicates the Rab27B Effector Protein Slac2-b in Inherited Skin Fragility. Am J Hum Genet 91:1115-21 (2012). PubMed: 23176819