Overview

  • Product name
  • Description
    Mouse polyclonal to RAB28
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Recombinant fragment
    Predicted to work with: Human
  • Immunogen

    Full length RAB28 protein (Human)

  • Positive control
    • transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 26 kDa).

Target

  • Relevance
    RAB28 belongs to the small GTPase superfamily. There are two isoforms. Isoform S is detected in most tissues investigated (cortex, liver, kidney, skeletal muscle, adipose tissue, testis and urothelium), whereas isoform L is predominant in testis.
  • Cellular localization
    Cell membrane; Lipid-anchor; Cytoplasmic side.
  • Database links
  • Alternative names
    • MGC41862 antibody
    • Rab26 antibody
    • RAB28, member RAS oncogene family antibody
    • Ras related protein Rab 28 antibody
    • Ras related protein Rab28 antibody
    see all

Images

  • All lanes : Anti-RAB28 antibody (ab67085) at 1/500 dilution

    Lane 1 : RAB28 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg/ml per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 26 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)

References

ab67085 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

ab67077 is actually a fragment of Rab28. I have updated the datasheet. Apologies for the confusion. This is why different bands sizes for immunogen transfected samples are seen. Both antibodies should be able to recognize both isoforms if present.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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