Overview

  • Product name
    Anti-RAB2B antibody
  • Description
    Rabbit polyclonal to RAB2B
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Zebrafish
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1-186 of RAB2B (Human)

  • Positive control
    • A431, H1299, HeLaS3, HepG2, MOLT4 or Raji lysates HeLa cells (IF)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Required for protein transport from the endoplasmic reticulum to the Golgi complex.
  • Tissue specificity
    Expressed in kidney, prostate, lung, liver, thymus, colon, pancreas, and skeletal muscle, and low levels in placenta. Not detected in heart, brain, spleen, testis, ovary, small intestine and leukocyte.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization
    Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ14824 antibody
    • GTP binding protein RAB2B antibody
    • GTP-binding protein RAB2B antibody
    • RAB2B antibody
    • RAB2B member RAS oncogene family antibody
    • RAB2B_HUMAN antibody
    • RAS family, member RAB2B antibody
    • ras related protein Rab 2B antibody
    • ras related protein Rab2B antibody
    • Ras-related protein Rab-2B antibody
    see all

Images

  • Anti-RAB2B antibody (ab95952) at 1/500 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 24 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab95952 at 1:200 dilution (1) and merged with DNA probe (2).

References

ab95952 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (Neural Precursor)
Permeabilization
Yes - 0.01% Triton X-100
Specification
Neural Precursor
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

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Verified customer

Submitted Oct 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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