Overview

  • Product nameAnti-RAB30 antibody
    See all RAB30 primary antibodies
  • Description
    Mouse monoclonal to RAB30
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant full length human RAB30 protein (AAH14213) with tag.

  • Positive control
    • Recombinant tagged full length human RAB30.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 23 kDa.

    This antibody has only been tested in WB against the recombinant protein used as immunogen. We have no data on the detection of endogenous protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5033421K01Rik antibody
      • AI323892 antibody
      • OTTMUSP00000025677 antibody
      • OTTMUSP00000025678 antibody
      • RAB 30 antibody
      • Rab30 antibody
      • RAB30 member RAS oncogene family antibody
      • RAB30_HUMAN antibody
      • Ras related protein Rab 30 antibody
      • Ras-associated protein Rab30 antibody
      • Ras-related protein Rab-30 antibody
      • Rsb30 antibody
      see all

    Anti-RAB30 antibody images

    • Anti-RAB30 antibody (ab76622) at 1 µg/ml + Recombinant tagged full length human RAB30 at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

      Predicted band size : 23 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)

    References for Anti-RAB30 antibody (ab76622)

    ab76622 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (A549 lung epithelial)
    Specification A549 lung epithelial
    Fixative 4% formaldehyde
    Permeabilization Yes - 0.2% triton x100 in PBS
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 18°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 16 2011

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (A549 lung epithelial)
    Loading amount 20000 cells
    Specification A549 lung epithelial
    Treatment siRNA treated
    Gel Running Conditions Reduced Denaturing (15%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 18°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 16 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"