Overview

  • Product name
    Anti-RAB33B antibody
  • Description
    Rabbit polyclonal to RAB33B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 39-229 of Human RAB33B (Uniprot ID Q9H082).

  • Positive control
    • NT2D1, IMR32, U87-MG, MCF7 and mouse brain whole cell lysates, Human gastric carcinoma and Human muscle tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab155679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

Target

Images

  • All lanes : Anti-RAB33B antibody (ab155679) at 1/1000 dilution

    Lane 1 : U87-MG whole cell lysate
    Lane 2 : MCF7 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 26 kDa



    12% SDS PAGE
  • Anti-RAB33B antibody (ab155679) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 26 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling RAB33B with ab155679 antibody at 1/500.
  • Immunohistochemical analysis of paraffin embedded Human muscle tissue labeling RAB33B with ab155679 antibody at 1/500.

References

ab155679 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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