The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration. PubMed: 23115302
FunctionIn the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
Cellular localizationCell membrane. Membrane > clathrin-coated pit. Cytoplasmic vesicle > clathrin-coated vesicle. Endosome. Melanosome. Present on sorting endosomes and endosomal tubules. Tends to be enriched in PIP2-positive cell membrane domains. During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Ras related protein rab 1c (GTP binding protein ray) antibody
Ras related protein Rab 1C antibody
Ras related protein Rab35 antibody
Ras-related protein Rab-1C antibody
Ras-related protein Rab-35 antibody
Anti-RAB35 antibody images
Immunohistochemistry (Frozen sections) - Anti-RAB35 antibody (ab105762)Image from Kley RA et al., Mol Cell Proteomics, 215-27, October 31, 2012. Fig 2.; doi: 10.1074/mcp.M112.023176. Epub 2012 Oct 31.
Immunohistochemical analysis of frozen serial sections of skeletal muscle biopsies from Human filaminopathy patients, staining RAB35 with ab105762.
Sections were incubated overnight at 4°C with primary antibody (1/500) and washed three times with PBS for 5 min. Subsequently, sections were DyLight®488-conjugated anti-rabbit IgG (1/1000).
Western blot - Anti-RAB35 antibody (ab105762)
Anti-RAB35 antibody (ab105762) (0.2-1 µg/ml) + Human Liver
Predicted band size : 23 kDa
References for Anti-RAB35 antibody (ab105762)
This product has been referenced in:
Kley RA et al. A combined laser microdissection and mass spectrometry approach reveals new disease relevant proteins accumulating in aggregates of filaminopathy patients. Mol Cell Proteomics12:215-27 (2013).
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