The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionMay be involved in melanosomal transport and docking. Involved in the proper sorting of TYRP1.
Tissue specificityExpressed in melanocytes.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
Post-translational modificationsAlthough at least one in vitro system can process and methylate the prenylated C-terminal, in an in vitro system that normally express Rab-38 and in vivo the prenylated C-terminal is not proteolytically processed and not methylated.
Predicted band size : 24 kDa Western blot analysis of HeLa (lane 1) and NIHT3 (lane 2) cell lysates labeling RAB38 with ab86542 at 1:1000. A HRP-conjugated Goat anti-rabbit (1:1000) was used as the secondary antibody.
Western blot - RAB38 antibody (ab86542)
Anti-RAB38 antibody (ab86542) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution