Overview

  • Product name
    Anti-RAB39B antibody [EPR9651]
    See all RAB39B primary antibodies
  • Description
    Rabbit monoclonal [EPR9651] to RAB39B
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human RAB39B.
    Database link: Q96DA2

  • Positive control
    • WB: Human fetal brain tissue lysate and U87-MG and SH-SY5Y cell lysates. ICC/IF: SH-SY5Y cells. Flow Cyt: U87-MG cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab154826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 25 kDa.

For unpurified use at 1/100.

ICC/IF 1/200.

For unpurified use at 1/10.

Flow Cyt 1/210.

For unpurified use at 1/9.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Relevance
      RAB39B is a member of the Rab family of proteins. Rab proteins are small GTPases that are involved in vesicular trafficking.
    • Cellular localization
      Cell membrane; Lipid-anchor; Cytoplasmic side
    • Database links
    • Alternative names
      • 6330580M05Rik antibody
      • MRX72 antibody
      • OTTHUMP00000024247 antibody
      • OTTMUSP00000020441 antibody
      • RAB39B, member RAS oncogene family antibody
      • Ras related protein Rab 39B antibody
      • RAS-associated protein RAB39B antibody
      • RP13-228J13.2 antibody
      • RP23-252K7.1 antibody
      see all

    Images

    • All lanes : Anti-RAB39B antibody [EPR9651] (ab154826) at 1/100 dilution (unpurified)

      Lane 1 : Human fetal brain tissue lysate
      Lane 2 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 25 kDa
      Observed band size : 25 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM/TBST.

    • Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling RAB39B with unpurified ab154826 at 1/10 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

    • Flow cytometry analysis of U87-MG cells labelling RAB39B with unpurified ab154826 (red) at 1/9. Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

    • All lanes : Anti-RAB39B antibody [EPR9651] (ab154826) at 1/2000 dilution (purified)

      Lane 1 : Human fetal brain tissue lysate
      Lane 2 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 25 kDa
      Observed band size : 25 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM/TBST.

    • Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling RAB39B with purified ab154826 at 1/200 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

    • Flow cytometry analysis of U87-MG cells labelling RAB39B with purified ab154826 (red) at 1/210. Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

    • All lanes : Anti-RAB39B antibody [EPR9651] (ab154826) at 1/1000 dilution (unpurified)

      Lane 1 : Fetal brain lysate
      Lane 2 : U87-MG cell lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 25 kDa

    References

    This product has been referenced in:

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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