Overview

  • Product name
  • Description
    Rabbit polyclonal to Rab3A
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Cynomolgus monkey
  • Immunogen

    Synthetic peptide:

    C-KQGPQLSDQQVPPHQD

    conjugated to KLH, corresponding to amino acids 202-217 of Human Rab3A (NP_002857.1).

  • Positive control
    • Human Brain, Cerebellum and Cortex tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage buffer
    pH: 8.20
    Constituents: 0.95% Sodium borate, 0.62% Boric Acid, 0.44% Sodium chloride, 0.15% EDTA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with 118863ab at 10 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with 118863 at 10 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • All lanes : Anti-Rab3A antibody (ab118863) at 1 µg/ml

    Lane 1 : HS67 Lysate
    Lane 2 : Mouse Brain Lysate
    Lane 3 : Rat Brain Lysate

    Lysates/proteins at 20 µg per lane.


    Performed under reducing conditions.

    Predicted band size : 24 kDa

References

ab118863 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab118863.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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