Overview

  • Product nameAnti-RAB3B antibody
    See all RAB3B primary antibodies
  • Description
    Mouse monoclonal to RAB3B
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: IKTYSWDNAQ VILVGNKCDM EEERVVPTEK GQLLAEQLGF DFFEASAKEN ISVRQAFERL VDAICDKMSD SLDTDPSMLG SSKNTRLSDT PPLLQQNCSC , corresponding to amino acids 120-219 of Human RAB3B

Properties

Applications

Our Abpromise guarantee covers the use of ab55655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 25 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionProtein transport. Probably involved in vesicular traffic.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain antigen RAB3B antibody
    • RAB3B antibody
    • RAB3B member RAS oncogene family antibody
    • RAB3B_HUMAN antibody
    • ras related protein Rab 3B antibody
    • Ras-related protein Rab-3B antibody
    see all

Anti-RAB3B antibody images

  • RAB3B antibody (ab55655) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human pancreas.


  • Predicted band size : 25 kDa
    RAB3B antibody (ab55655) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.
  • Overlay histogram showing HepG2 cells stained with ab55655 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55655, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-RAB3B antibody (ab55655)

ab55655 has not yet been referenced specifically in any publications.

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