Overview

  • Product name
  • Description
    Rabbit polyclonal to Rab3C
  • Tested applications
    Suitable for: WB, IP, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Rat Rab3C aa 9-26.
    Sequence:

    MASAQDARFGQKDSSDQN


    (Peptide available as ab4952)

  • Positive control
    • AtT20 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab3336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Can be blocked with Rab3C peptide (ab4952).
IP Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19786578

Target

Anti-Rab3C antibody images

  • Western blot of Rab3C on AtT20 cell lysate using ab3336.

References for Anti-Rab3C antibody (ab3336)

This product has been referenced in:

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spinal Cord)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Spinal Cord
Blocking step
1%BSA+10%FBS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Methanol
Username

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Verified customer

Submitted Oct 03 2016

Application
Western blot
Sample
Human Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 08 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 08 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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