Overview

  • Product nameAnti-RAB3GAP2 antibody
    See all RAB3GAP2 primary antibodies
  • Description
    Rabbit polyclonal to RAB3GAP2
  • Specificityab110462 detects endogenous levels of total RAB3GAP2 protein.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal region of Human RAB3GAP2 (UniProt ID: Q9H2M9).

  • Positive control
    • Human breast carcinoma tissue; extracts from COS cells

Properties

Applications

Our Abpromise guarantee covers the use of ab110462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 155 kDa (predicted molecular weight: 156 kDa).
IHC-P 1/50 - 1/100. Antigen retrieval is heat-mediated, citrate buffer pH 6.0. A pressure cooker was used for about 5 minutes.

Target

  • RelevanceRAB3GAP2 is a regulatory subunit of a GTPase activating protein that has specificity for Rab3 subfamily (RAB3A, RAB3B, RAB3C and RAB3D). Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Rab3 GTPase-activating complex specifically converts active Rab3-GTP to the inactive form Rab3-GDP. It is required for normal eye and brain development and may participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non-synaptic vesicular release of neurotransmitters.
  • Cellular localizationCytoplasmic. Note: In neurons, it is enriched in the synaptic soluble fraction.
  • Database links
  • Alternative names
    • DKFZp434D245 antibody
    • FLJ14579 antibody
    • KIAA0839 antibody
    • p150 antibody
    • Rab3 GAP p150 antibody
    • Rab3 GAP regulatory subunit antibody
    • Rab3 GAP150 antibody
    • Rab3 GTPase activating protein 150 kDa subunit antibody
    • Rab3 GTPase activating protein non catalytic subunit antibody
    • RAB3 GTPase activating protein subunit 2 (non catalytic) antibody
    • RAB3 GTPase activating protein subunit 2 antibody
    • RAB3GAP150 antibody
    • RGAP iso antibody
    • RP11 568G11.1 antibody
    see all

Anti-RAB3GAP2 antibody images

  • All lanes : Anti-RAB3GAP2 antibody (ab110462) at 1/500 dilution

    Lane 1 : Extracts from COS cells
    Lane 2 : Extracts from COS cells, treated with the synthesized peptide

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 156 kDa
    Observed band size : 155 kDa (why is the actual band size different from the predicted?)
  • ab110462, at a 1/50 dilution, staining RAB3GAP2 in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the synthesized peptide.

References for Anti-RAB3GAP2 antibody (ab110462)

ab110462 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"