Overview

  • Product nameAnti-RAB3IP antibody
    See all RAB3IP primary antibodies
  • Description
    Rabbit polyclonal to RAB3IP
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (PDLLGVYESG TQEQTTSPSV IYRPHPSALS SVPIQANALD VSELPTQPVY) of Human RAB3IP (NP_783322).

  • Positive control
    • 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab90732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionGuanine nucleotide exchange factor (GEF) which may activate RAB8A and RAB8B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Mediates the release of GDP from RAB8A and RAB8B but not from RAB3A or RAB5. Modulates actin organization and promotes polarized transport of RAB8A-specific vesicles to the cell surface. Together with RAB11A, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis.
  • Tissue specificityExpressed in brain, kidney, heart, pancreas and placenta. Not detected in skeletal muscle or liver.
  • Sequence similaritiesBelongs to the SEC2 family.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Predominantly cytoplasmic but a small proportion colocalizes with SSX2 in the nucleus. Activation of protein kinase C results in redistribution to the periphery of lamellipodia. In the cytoskeleton, localizes to cortical actin.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gtpat12 antibody
    • Rab-3A-interacting protein antibody
    • RAB3A interacting protein antibody
    • RAB3A interacting protein (rabin3) antibody
    • RAB3A interacting protein antibody
    • RAB3A-interacting protein antibody
    • RAB3I_HUMAN antibody
    • Rab3ip antibody
    • Rabin 3 antibody
    • Rabin-3 antibody
    • RABIN3 antibody
    • RABIN3, rat, homolog of antibody
    • Rabin8 antibody
    • SSX2 interacting protein antibody
    • SSX2 interacting protein antibody
    • SSX2-interacting protein antibody
    see all

Anti-RAB3IP antibody images

  • Primary Antibody Dilution : 1:200. Secondary Antibody : Goat anti-rabbit Alexafluor 568. Secondary Antibody Dilution : 1:200. Color/Signal Descriptions : RAB3IP: Red CtBp: Green DAPI: Blue. Gene Name : RAB3IP.
  • Primary Antibody Dilution : 1:200. Secondary Antibody : Goat anti-rabbit Alexafluor 568. Secondary Antibody Dilution : 1:200. Color/Signal Descriptions : RAB3IP: Red CtBp: Green DAPI: Blue. Gene Name : RAB3IP.
  • Primary Antibody Dilution : 1:200. Secondary Antibody : Goat anti-rabbit Alexafluor 568. Secondary Antibody Dilution : 1:200. Color/Signal Descriptions : RAB3IP: Red CtBp: Green DAPI: Blue. Gene Name : RAB3IP.
  • Anti-RAB3IP antibody (ab90732) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Gel concentration: 12%

References for Anti-RAB3IP antibody (ab90732)

ab90732 has not yet been referenced specifically in any publications.

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