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Recombinant fusion protein (Human) expressed in E.coli.
Our Abpromise guarantee covers the use of ab13252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 17547697|
|WB||1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 25.6 kDa).|
|ICC||1/200 - 1/500.|
ab13252 Rabbit polyclonal to Rab4 was used in fixed primary cultures of rat hippocampal neurons (embryonic day 18) at a dilution of 1/200. A secondary goat anti-rabbit antibody was used for detection (Alexa Fluor 488; 1/200; green). Confocal microscopy revealed typically punctuate immunofluorescence expected of Rab4 in these neurons. The red colour labelling is that of monoclonal mouse anti-
Predicted band size : 25.6 kDa