Overview

  • Product nameAnti-Rab4 antibody
    See all Rab4 primary antibodies
  • Description
    Rabbit polyclonal to Rab4
  • SpecificityThis antibody is very specific for Rab4. To our knowledge this antibody does not cross-react with other Rab proteins. However, as there are dozens of different proteins in the Rab family, not every single one has been tested for cross-reactivity with this antibody.
  • Tested applicationsSuitable for: ICC/IF, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Recombinant fusion protein (Human) expressed in E.coli.

  • Positive control
    • Mouse or Rat Brain Tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab13252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 17547697
WB 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 25.6 kDa).
ICC 1/200 - 1/500.

Target

Anti-Rab4 antibody images

  • ab13252 Rabbit polyclonal to Rab4 was used in fixed primary cultures of rat hippocampal neurons (embryonic day 18) at a dilution of 1/200. A secondary goat anti-rabbit antibody was used for detection (Alexa Fluor 488; 1/200; green). Confocal microscopy revealed typically punctuate immunofluorescence expected of Rab4 in these neurons. The red colour labelling is that of monoclonal mouse anti-

    Anti-Rab4 antibody (ab13252) at 1/1000 dilution + Lysates prepared from rat brain tissue

    Predicted band size : 25.6 kDa

References for Anti-Rab4 antibody (ab13252)

This product has been referenced in:

See all 12 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HRMEC)
Permeabilization Yes - 0.1% Triton in PBS
Specification HRMEC
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10%
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 23 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (COS7)
Permeabilization Yes - 0.2%Triton
Specification COS7
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 07 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (bEnd.3)
Permeabilization Yes - 0.1% Triton in PBS%
Specification bEnd.3
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 03 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293T cells)
Loading amount 25 µg
Specification HEK293T cells
Gel Running Conditions Reduced Denaturing (4-12% SDS-PAGE gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jan 28 2013

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab2900.

To check the status of the order please contact ou...

Read More

Thank you for contacting Abcam.

The antibody was raised against the whole molecule of human Rab4 and was expressed in E.coli.

Please let me know if there is anything else I can help you with.

Thank you for your response.

My colleague is out of office this week and she has asked me to look after her customers.

There seems to be a faint band at 15 kDa and another stronger one at 60 kDa in the Western blot image you have se...

Read More

Thank you for your response.

My colleague Kate is out of office this week and she has asked me to look after her colleagues.

I understand that this vial was a free of charge replacement. Could you please summarize if the problem wit...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More

Thank you for taking the time to complete our questionnaire. The details you have kindly provided will provide us with vital information for our monitoring of product quality

I appreciate the time you have spent in the laboratory and underst...

Read More

1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"