Overview

  • Product nameAnti-Rab4 antibody
    See all Rab4 primary antibodies
  • Description
    Mouse monoclonal to Rab4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-219 of Human Rab4

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab55656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 24 kDa.

Target

  • FunctionProtein transport. Probably involved in vesicular traffic.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
  • Post-translational
    modifications
    Phosphorylated by CDK1 kinase during mitosis.
  • Cellular localizationMembrane. Cytoplasm. Generally associated with membranes. Cytoplasmic when phosphorylated by CDK1.
  • Information by UniProt
  • Database links
  • Alternative names
    • HRES 1 / RAB4 antibody
    • Oncogene RAB4 antibody
    • Rab 4 antibody
    • RAB 4A antibody
    • RAB4 member RAS oncogene family antibody
    • Rab4a antibody
    • RAB4A member RAS oncogene family antibody
    • RAB4A_HUMAN antibody
    • Ras related protein Rab 4A antibody
    • Ras related protein Rab4A antibody
    • Ras-related protein Rab-4A antibody
    see all

Anti-Rab4 antibody images



  • Predicted band size : 24 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Rab4 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Human fetal brain lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab55656 observed at 24 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab55656 was shown to specifically react with Rab4 when Rab4 knockout samples were used. Wild-type and Rab4 knockout samples were subjected to SDS-PAGE. ab55656 and ab181602 (loading control to GAPDH) were diluted 1 µg/mL and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.



  • Predicted band size : 24 kDa
    Rab4 antibody (ab55656) at 1ug/lane + HeLa cell lysate at 25ug/lane.

References for Anti-Rab4 antibody (ab55656)

This product has been referenced in:
  • Potokar M  et al. Rab4 and Rab5 GTPase are required for directional mobility of endocytic vesicles in astrocytes. Glia 60:594-604 (2012). Read more (PubMed: 22279005) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab55656.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"