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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Overview
Product name
Anti-Rab4 antibody
See all Rab4 products (11) ...
Description
Rabbit polyclonal to Rab4
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Sheep, Cow, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Rab4.
(Peptide available as ab88190.)
Positive control
This antibody gave a positive signal in the following lysates: Human Brain Tissue; Human Brain Tissue Membrane Extract; Mouse Brain Tissue; Rat Brain Tissue; SHSY5Y Whole Cell; SKNSH Whole Cell; HepG2 Whole Cell; HeLa Whole Cell.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab78970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
Target
Function
Protein transport. Probably involved in vesicular traffic.
Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Post-translational
modifications
Phosphorylated by CDK1 kinase during mitosis.
Cellular localization
Membrane. Cytoplasm. Generally associated with membranes. Cytoplasmic when phosphorylated by CDK1.
Target information above from: UniProt accessionP20338
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Oncogene RAB4 antibody
- OTTHUMP00000042824 antibody
- Rab 4 antibody
see all
Database links
- Entrez Gene: 5867 Human
- Entrez Gene: 19341 Mouse
- Entrez Gene: 25532 Rat
- Entrez Gene: 403031 Zebrafish
- Omim: 179511 Human
- SwissProt: P20338 Human
- SwissProt: P56371 Mouse
- SwissProt: P05714 Rat
see all
Anti-Rab4 antibody images:
Western blot - Rab4 antibody (ab78970)
All lanes : Anti-Rab4 antibody (ab78970) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Brain (Human) Membrane Lysate - adult normal tissue (ab29456)
Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 4 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 24 kDa
Observed band size : 24 kDa
Additional bands at : 150 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
Immunocytochemistry/ Immunofluorescence - Rab4 antibody (ab78970)
ICC/IF image of ab78970 stained HeLa cells. The cells were 4% XPFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78970, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2, and MCF-7 cells at 5µg/ml.
Western blot - Anti-Rab4 antibody (ab78970)
All lanes : Anti-Rab4 antibody (ab78970) at 1 µg/ml
Lane 1 : E16 Ms Embryo Brain Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Predicted band size : 24 kDa
Observed band size : 24 kDa
Exposure time : 2 minutes
References for Anti-Rab4 antibody (ab78970)
ab78970 has not yet been referenced specifically in any publications.
Publishing research using ab78970? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"