Validated using a knockout cell line

Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab108974)


  • Product name
    Anti-Rab4 antibody [EPR3042] - Early Endosome Marker
    See all Rab4 primary antibodies
  • Description
    Rabbit monoclonal [EPR3042] to Rab4 - Early Endosome Marker
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Rab4.

  • Positive control
    • MCF7, PC12 and fetal brain cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:

    Anti-Rab4 antibody - Early Endosome Marker (HRP) [EPR3042] (ab199205)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab108974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
Flow Cyt 1/150. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    Anti-Rab4 antibody [EPR3042] - Early Endosome Marker images

    • Predicted band size : 24 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Rab4 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Human fetal brain cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab108974 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab108974 was shown to recognize Rab4 when Rab4 knockout samples were used, along with additional cross-reactive bands. Wild-type and Rab4 knockout samples were subjected to SDS-PAGE. ab108974 and ab8245 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab108974) at 1/2000 dilution

      Lane 1 : MCF7 cell lysates
      Lane 2 : PC12 cell lysates
      Lane 3 : Fetal brain cell lysates

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 24 kDa
      Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of Rab4 in HeLa cells, using ab108974 at a 1/100 dilution.

    References for Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab108974)

    ab108974 has not yet been referenced specifically in any publications.

    Product Wall

    Deux anticorps semblent intéressants pour la détection de Rab4 chez la Drosophile:
    Le premier est un polyclonal de lapin dont l'immunogène présente 86% d'homologie avec la protéine d'intéret (voir fichier pdf ci-joint "ab78970 vs Drosophila"). La r...

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    Thank you for getting back to me with that information.

    I am sorry, I did miss understand you, I thought you were using tissue, not cell lines. This does make the band at 50 kDa unexpected, although I wouldsay that a"no-primary" con...

    Read More

    Thank you for the swift reply.

    The extra details have made it much easier to understand the problems you have been encountering. Although there is a faint band at the expected size (˜25 kDa), as you have described, the non-specific band at ˜4...

    Read More

    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with the anti-Rab4 antibody (ab108974). We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. ...

    Read More


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